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PMID: 16880513 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.

Molecular and cellular biology ·Vol. 26 ·No. 16 ·2006-08-00 ·Pages 6016-23

Boon KL, Auchynnikava T, Edwalds-Gilbert G, Barrass JD, Droop AP, Dez C, Beggs JD

Abstract

The Ntr1 and Ntr2 proteins of Saccharomyces cerevisiae have been reported to interact with proteins involved in pre-mRNA splicing, but their roles in the splicing process are unknown. We show here that they associate with a postsplicing complex containing the excised intron and the spliceosomal U2, U5, and U6 snRNAs, supporting a link with a late stage in the pre-mRNA splicing process. Extract from cells that had been metabolically depleted of Ntr1 has low splicing activity and accumulates the excised intron. Also, the level of U4/U6 di-snRNP is increased but those of the free U5 and U6 snRNPs are decreased in Ntr1-depleted extract, and increased levels of U2 and decreased levels of U4 are found associated with the U5 snRNP protein Prp8. These results suggest a requirement for Ntr1 for turnover of the excised intron complex and recycling of snRNPs. Ntr1 interacts directly or indirectly with the intron release factor Prp43 and is required for its association with the excised intron. We propose that Ntr1 promotes release of excised introns from splicing complexes by acting as a spliceosome receptor or RNA-targeting factor for Prp43, possibly assisted by the Ntr2 protein.

MeSH Terms
DEAD-box RNA Helicases Introns/genetics Protein Binding RNA Helicases/metabolism RNA Splicing/genetics Ribonucleoproteins, Small Nuclear/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism Spliceosomes/metabolism
Chemicals
Ntr2 protein, S cerevisiae Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae Proteins Spp382 protein, S cerevisiae PRP43 protein, S cerevisiae DEAD-box RNA Helicases RNA Helicases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Boon Kum-Loong
Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, United Kingdom.
Auchynnikava Tatsiana
Edwalds-Gilbert Gretchen
Barrass J David
Droop Alastair P
Dez Christophe
Beggs Jean D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.02347-05
Published
2006-08-00
Pages
6016-23
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1592814
Subset
IM
Grants
NIGMS NIH HHS · R15 GM072622 · United States
Wellcome Trust · 067311 · United Kingdom
NIGMS NIH HHS · R15 GM/072622 · United States
External Links
PubMed source DOI: 10.1128/MCB.02347-05 PMC Full Text View journal details
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