Home LiteratureArticle Details
PMID: 178597 Published · ppublish English Journal Article

Purification of hepatitis A antigen from feces and detection of antigen and antibody by immune adherence hemagglutination.

Infection and immunity ·Vol. 13 ·No. 3 ·1976-03-00 ·Pages 898-908

Moritsugu Y, Dienstag JL, Valdesuso J, Wong DC, Wagner J, Routenberg JA, Purcell RH

Abstract

Hepatitis A antigen (HA Ag) was purified from feces collected during acute illness from patients with naturally occurring viral hepatitis, type A. Positive fecal specimens were identified by immune electron microscopy, but for detection of HA Agduring purification immune adherence hemagglutination (IAHA) and microtiter solid-phase radioimmunoassay were used. Isopycnic banding in cesium chloride, rate-zonal separation in sucrose, and preparative zonal electrophoresis were used in various combinations for successive purification, and the purified antigen was successfully used in a test for antibody by IAHA. Seronconversions to HA Ag were demonstrated by IAHA in 20 instances of hepatitis A virus infection, but in none of six cases of type B hepatitis or three cases of post-transfusion hepatitis unrelated to heaptitis A or B viruses, nor in two individuals without hepatitis. In addition, the temporal pattern of antibody development during type A hepatitis was studied in serial sera from an experimentally infected chimpanzee. Antibody titers by IAHA correlated well with antibody ratings determined by immune electron microscopy.

MeSH Terms
Antibodies, Viral/analysis Antigens, Viral/analysis,isolation & purification Centrifugation, Density Gradient Centrifugation, Isopycnic Electrophoresis Feces/microbiology Hemagglutination Tests Hepatitis B Antigens/immunology Hepatovirus/ultrastructure Humans Immune Adherence Reaction In Vitro Techniques Radioimmunoassay
Chemicals
Antibodies, Viral Antigens, Viral Hepatitis B Antigens
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Moritsugu Y
Dienstag J L
Valdesuso J
Wong D C
Wagner J
Routenberg J A
Purcell R H
References (27)
27 references, click to expand
  1. Hepatitis A: report of a common-source outbreak with recovery of a possible etiologic agent. II. Laboratory studies.
    J Infect Dis. 1975 Feb;131(2):167-71 PMID: 1117190
  2. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  3. Viral hepatitis, type A. Identification by specific complement fixation and immune adherence tests.
    N Engl J Med. 1975 May 29;292(22):1141-3 PMID: 164627
  4. Physical, chemical and morphologic dimensions of human hepatitis A virus strain CR326 (38578).
    Proc Soc Exp Biol Med. 1975 Feb;148(2):532-9 PMID: 164674
  5. A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology.
    Proc Soc Exp Biol Med. 1975 Apr;148(4):962-9 PMID: 165548
  6. Specific immune adherence assay for human hepatitis A antibody application to diagnostic and epidemiologic investigations.
    Proc Soc Exp Biol Med. 1975 May;149(1):254-61 PMID: 167376
  7. Experimental infection of chimpanzees with hepatitis A virus.
    J Infect Dis. 1975 Nov;132(5):532-45 PMID: 171320
  8. Etiological spectrum of post-transfusion hepatitis.
    Gastroenterology. 1975 Dec;69(6):1278-85 PMID: 172400
  9. Foodhandler-associated outbreak of hepatitis type A. An immune electron microscopic study.
    Ann Intern Med. 1975 Nov;83(5):647-50 PMID: 173217
  10. A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody.
    J Immunol. 1976 Feb;116(2):349-56 PMID: 175117
  11. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis.
    J Virol. 1972 Nov;10(5):1075-81 PMID: 4117963
  12. Infectious hepatitis. Evidence for two distinctive clinical, epidemiological, and immunological types of infection.
    JAMA. 1967 May 1;200(5):365-73 PMID: 4164595
  13. Specific neutralization of human hepatitis type A in marmoset monkeys.
    Nature. 1973 Jun 15;243(5407):419-20 PMID: 4355234
  14. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness.
    Science. 1973 Dec 7;182(4116):1026-8 PMID: 4356028
  15. The relationship between a 27-nm virus-like particle and hepatitis A as demonstrated by immune electron microscopy.
    Intervirology. 1974;4(2):110-8 PMID: 4448627
  16. Recovery of hepatitis agents in the marmoset from human cases occurring in Costa Rica.
    Proc Soc Exp Biol Med. 1973 Jan;142(1):276-82 PMID: 4630292
  17. Etiologic relationship of marmoset-propagated CR326 hepatitis A virus to hepatitis in man.
    Proc Soc Exp Biol Med. 1973 Apr;142(4):1257-67 PMID: 4632938
  18. A major epidmeic of infectious hepatitis in an institution for the mentally retarded.
    Am J Epidemiol. 1973 Sep;98(3):199-215 PMID: 4741841
  19. Buoyant density of the hepatitis A virus-like particle in cesium chloride.
    J Virol. 1974 Jun;13(6):1412-4 PMID: 4833615
  20. Studies on the transmission of human viral hepatitis to marmoset monkeys. I. Transmission of disease, serial passages, and description of liver lesions.
    J Exp Med. 1967 Apr 1;125(4):673-88 PMID: 4960092
  21. Hepatitis in marmosets: induction of disease with coded specimens from a human volunteer study.
    Science. 1969 Aug 22;165(3895):816-7 PMID: 4978752
  22. The Holy Cross college football team hepatitis outbreak.
    JAMA. 1972 Feb;219(6):706-8 PMID: 5066695
  23. Frequency of antibody to hepatitis-associated antigen as measured by a new radioimmunoassay technique.
    J Immunol. 1971 May;106(5):1166-71 PMID: 5103349
  24. Detection of hepatitis A viral antigen by radioimmunoassay.
    J Immunol. 1975 Nov;115(5):1464-6 PMID: 51893
  25. Relationship of hepatitis A antigen to viral hepatitis.
    Am J Med Sci. 1975 Jul-Aug;270(1):61-71 PMID: 53012
  26. Viral hepatitis. Clinical and tissue culture studies.
    JAMA. 1970 Nov 9;214(6):1041-6 PMID: 5536247
  27. Detection of Australia antigen by means of immune adherence haemagglutination test.
    Vox Sang. 1971 Feb;20(2):178-81 PMID: 5581703
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.13.3.898-908.1976
Published
1976-03-00
Pages
898-908
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC420693
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.13.3.898-908.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2