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PMID: 17850745 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution.

Structure (London, England : 1993) ·Vol. 15 ·No. 9 ·2007-09-00 ·Pages 1053-64

Chiu PL, Pagel MD, Evans J, Chou HT, Zeng X, Gipson B, Stahlberg H, Nimigean CM

Abstract

The gating ring of cyclic nucleotide-modulated channels is proposed to be either a two-fold symmetric dimer of dimers or a four-fold symmetric tetramer based on high-resolution structure data of soluble cyclic nucleotide-binding domains and functional data on intact channels. We addressed this controversy by obtaining structural data on an intact, full-length, cyclic nucleotide-modulated potassium channel, MloK1, from Mesorhizobium loti, which also features a putative voltage-sensor. We present here the 3D single-particle structure by transmission electron microscopy and the projection map of membrane-reconstituted 2D crystals of MloK1 in the presence of cAMP. Our data show a four-fold symmetric arrangement of the CNBDs, separated by discrete gaps. A homology model for full-length MloK1 suggests a vertical orientation for the CNBDs. The 2D crystal packing in the membrane-embedded state is compatible with the S1-S4 domains in the vertical "up" state.

MeSH Terms
Amino Acid Sequence Crystallography Cyclic AMP/metabolism Microscopy, Electron, Transmission Models, Molecular Molecular Sequence Data Potassium Channels/chemistry Protein Conformation Rhizobium/chemistry Sequence Homology, Amino Acid
Chemicals
Potassium Channels Cyclic AMP
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chiu Po-Lin
Molecular and Cellular Biology, College of Biological Sciences, University of California-Davis, 1 Shields Avenue, Davis, CA 95616, USA.
Pagel Matthew D
Evans James
Chou Hui-Ting
Zeng Xiangyan
Gipson Bryant
Stahlberg Henning
Nimigean Crina M
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Article Info
Journal
Structure (London, England : 1993)
Abbr.
Structure
ISSN
0969-2126
DOI
10.1016/j.str.2007.06.020
Published
2007-09-00
Pages
1053-64
Language
English
Region
United States
NLM ID
101087697
PMCID
PMC2000844
Subset
IM
Grants
NIGMS NIH HHS · U54 GM074929-010006 · United States
NIGMS NIH HHS · U54 GM074929-020006 · United States
NIGMS NIH HHS · U54 GM074929-030006 · United States
NIGMS NIH HHS · R01 GM081653 · United States
NIGMS NIH HHS · R01 GM077560 · United States
NIGMS NIH HHS · U54 GM074929 · United States
NIGMS NIH HHS · R01 GM081653-01 · United States
Corrections
CommentIn
PMID: 17850739
External Links
PubMed source DOI: 10.1016/j.str.2007.06.020 PMC Full Text View journal details
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