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PMID: 17715218 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins.

Journal of virology ·Vol. 81 ·No. 21 ·2007-11-00 ·Pages 11790-7

Coller KE, Lee JI, Ueda A, Smith GA

Abstract

How alphaherpesvirus capsids acquire tegument proteins remains a key question in viral assembly. Using pseudorabies virus (PRV), we have previously shown that the 62 carboxy-terminal amino acids of the VP1/2 large tegument protein are essential for viral propagation and when transiently expressed as a fusion to green fluorescent protein relocalize to nuclear capsid assemblons following viral infection. Here, we show that localization of the VP1/2 capsid-binding domain (VP1/2cbd) into assemblons is conserved in herpes simplex virus type 1 (HSV-1) and that this recruitment is specifically on capsids. Using a mutant virus screen, we find that the protein product of the UL25 gene is essential for VP1/2cbd association with capsids. An interaction between UL25 and VP1/2 was corroborated by coimmunoprecipitation from cells transiently expressing either HSV-1 or PRV proteins. Taken together, these findings suggest that the essential function of the VP1/2 carboxy terminus is to anchor the VP1/2 tegument protein to capsids. Furthermore, UL25 encodes a multifunctional capsid protein involved in not only encapsidation, as previously described, but also tegumentation.

MeSH Terms
Alphaherpesvirinae/chemistry,physiology Animals Capsid Cell Line Chlorocebus aethiops Gene Expression Regulation, Viral Immunoprecipitation Mutation Open Reading Frames Protein Binding Swine Vero Cells Viral Core Proteins/chemistry Viral Proteins/chemistry
Chemicals
Viral Core Proteins Viral Proteins DNA cleavage and packaging proteins, Herpesvirus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Coller Kelly Elizabeth
Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Lee Joy I-Hsuan
Ueda Aki
Smith Gregory Allan
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.01113-07
Published
2007-11-00
Epub
2007-00-22
Pages
11790-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC2168758
Subset
IM
Grants
NIAID NIH HHS · R01 AI056346 · United States
NIAID NIH HHS · T32 AI007476 · United States
NIAID NIH HHS · 1R05 AI 056346 · United States
NIAID NIH HHS · T32 AI 07476 · United States
External Links
PubMed source DOI: 10.1128/JVI.01113-07 PMC Full Text View journal details
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