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PMID: 16352544 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The pseudorabies virus VP1/2 tegument protein is required for intracellular capsid transport.

Journal of virology ·Vol. 80 ·No. 1 ·2006-01-00 ·Pages 201-9

Luxton GW, Lee JI, Haverlock-Moyns S, Schober JM, Smith GA

Abstract

Transport of capsids in cells is critical to alphaherpesvirus infection and pathogenesis; however, viral factors required for transport have yet to be identified. Here we provide a detailed examination of capsid dynamics during the egress phase of infection in Vero cells infected with pseudorabies virus. We demonstrate that the VP1/2 tegument protein is required for processive microtubule-based transport of capsids in the cytoplasm. A second tegument protein that binds to VP1/2, UL37, was necessary for wild-type transport but was not essential for this process. Both proteins were also required for efficient nuclear egress of capsids to the cytoplasm.

MeSH Terms
Animals Capsid Proteins/metabolism Chlorocebus aethiops Herpesvirus 1, Suid/physiology Protein Transport Vero Cells Viral Fusion Proteins/physiology Viral Structural Proteins/physiology Virion/physiology Virus Assembly/physiology
Chemicals
Capsid Proteins UL37 protein, Human herpesvirus 1 Viral Fusion Proteins Viral Structural Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Luxton G W Gant
Department of Microbiology-Immunology, Ward Bldg., Rm. 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Lee Joy I-Hsuan
Haverlock-Moyns Sarah
Schober Joseph Martin
Smith Gregory Allan
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.80.1.201-209.2006
Published
2006-01-00
Pages
201-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC1317523
Subset
IM
Grants
NIAID NIH HHS · R01 AI056346 · United States
NIAID NIH HHS · 1R01AI056346 · United States
External Links
PubMed source DOI: 10.1128/JVI.80.1.201-209.2006 PMC Full Text View journal details
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