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PMID: 17626073 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Epstein-Barr virus BHRF1 micro- and stable RNAs during latency III and after induction of replication.

Journal of virology ·Vol. 81 ·No. 18 ·2007-09-00 ·Pages 9967-75

Xing L, Kieff E

Abstract

Epstein-Barr virus (EBV) microRNAs miR-BHRF1-1, -2, and -3 have been detected in latency III-infected lymphoblasts, where they are encoded within EBNA transcripts (X. Cai, A. Schafer, S. Lu, J. P. Bilello, R. C. Desrosiers, R. Edwards, N. Raab-Traub, and B. R. Cullen, PLoS Pathog. 2:e23, 2006). In latency III-infected lymphoblasts, we have also identified a stable 1.3-kb RNA, which begins 3' to miR-BHRF1-1, includes the BHRF1 open reading frame, and ends near miR-BHRF1-2. This 1.3-kb RNA is the residue of Drosha cleavage of the BHRF1 microRNAs from EBNA transcripts. Early after induction of EBV replication in latency I-infected Akata lymphoblasts, BHRF1 spliced 1.4-kb mRNA accumulated along with low levels of miR-BHRF1-2 and -3 and a 0.9-kb Drosha or miR-BHRF1-2 cleavage product of BHRF1 mRNA. The turning on of latency III infection at 48 to 72 h after induction of EBV replication was associated with higher miR-BHRF1-1, -2, and -3 levels; accumulation of the 1.3-kb RNA residue in the nucleus; abundant BHRF1 spliced 1.4-kb mRNA in the cytoplasm; and more abundant 0.9-kb mRNA cleavage product in the cytoplasm. These findings implicate miR-BHRF1-2 in 3' cleavage of BHRF1 mRNA in the cytoplasm and Drosha in cleavage of latency III EBNA and EBV replication-associated BHRF1 transcripts in the nucleus.

MeSH Terms
Animals Base Sequence Cell Line, Tumor Cell Nucleus/metabolism,virology Cytoplasm/metabolism,virology Epstein-Barr Virus Nuclear Antigens/metabolism Herpesvirus 4, Human/physiology Humans Lymphocytes/metabolism,virology Mice Mice, Nude MicroRNAs/metabolism Molecular Sequence Data Open Reading Frames/physiology RNA Splicing/physiology RNA Stability/physiology RNA, Messenger/metabolism RNA, Viral/metabolism Ribonuclease III/metabolism Viral Proteins/metabolism Virus Latency/physiology Virus Replication/physiology
Chemicals
BHRF1 protein, Human herpesvirus 4 Epstein-Barr Virus Nuclear Antigens MicroRNAs RNA, Messenger RNA, Viral Viral Proteins DROSHA protein, human Ribonuclease III
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xing Li
Brigham and Women's Hospital, Harvard Medical School, Channing Laboratory, 181 Longwood Avenue, Boston, MA 02115-5804, USA.
Kieff Elliott
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.02244-06
Published
2007-09-00
Epub
2007-00-11
Pages
9967-75
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC2045418
Subset
IM
Grants
NCI NIH HHS · R01 CA047006 · United States
NCI NIH HHS · R35 CA047006 · United States
NCI NIH HHS · CA 47006 · United States
Databases
GENBANK
EF192979
External Links
PubMed source DOI: 10.1128/JVI.02244-06 PMC Full Text View journal details
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