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PMID: 17573477 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Identification of genes in Xanthomonas campestris pv. vesicatoria induced during its interaction with tomato.

Journal of bacteriology ·Vol. 189 ·No. 17 ·2007-09-00 ·Pages 6359-71

Tamir-Ariel D, Navon N, Burdman S

Abstract

Xanthomonas campestris pv. vesicatoria is the causal agent of bacterial spot disease of tomato and pepper. The disease process is interactive and very intricate and involves a plethora of genes in the pathogen and in the host. In the pathogen, different genes are activated in response to the changing environment to enable it to survive, adapt, evade host defenses, propagate, and damage the host. To understand the disease process, it is imperative to broaden our understanding of the gene machinery that participates in it, and the most reliable way is to identify these genes in vivo. Here, we have adapted a recombinase-based in vivo expression technology (RIVET) to study the genes activated in X. campestris pv. vesicatoria during its interaction with one of its hosts, tomato. This is the first study that demonstrates the feasibility of this approach for identifying in vivo induced genes in a plant pathogen. RIVET revealed 61 unique X. campestris pv. vesicatoria genes or operons that delineate a picture of the different processes involved in the pathogen-host interaction. To further explore the role of some of these genes, we generated knockout mutants for 13 genes and characterized their ability to grow in planta and to cause disease symptoms. This analysis revealed several genes that may be important for the interaction of the pathogen with its host, including a citH homologue gene, encoding a citrate transporter, which was shown to be required for wild-type levels of virulence.

MeSH Terms
DNA, Bacterial/chemistry,genetics Gene Deletion Gene Expression Profiling/methods Gene Expression Regulation, Bacterial Lycopersicon esculentum/microbiology Molecular Sequence Data Mutagenesis, Insertional Plant Diseases/microbiology Sequence Analysis, DNA Up-Regulation Virulence/genetics Xanthomonas campestris/genetics,growth & development,pathogenicity
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tamir-Ariel Dafna
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.
Navon Naama
Burdman Saul
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.00320-07
Published
2007-09-00
Epub
2007-00-15
Pages
6359-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1951904
Subset
IM
Databases
GENBANK
EF633111, EF633112, EF633113, EF633114, EF633115, EF633116, EF633117, EF633118, EF633119, EF633120, EF633121, EF633122, EF633123, EF633124, EF633125, EF633126, EF633127, EF633128, EF633129, EF633130, EF633131, EF633132, EF633133, EF633134, EF633135, EF633136, EF633137, EF633138, EF633139, EF633140, EF633141, EF633142, EF633143, EF633144, EF633145, EF633146, EF633147, EF633148, EF633149, EF633150, EF633151, EF633152, EF633153, EF633154, EF633155, EF633156, EF633157, EF633158, EF633159, EF633160, EF633161, EF633162, EF633163, EF633164, EF633165, EF633166, EF633167, EF633168, EF633169, EF633170, EF633171
External Links
PubMed source DOI: 10.1128/JB.00320-07 PMC Full Text View journal details
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