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PMID: 17567699 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Functionally distinct transmission of human immunodeficiency virus type 1 mediated by immature and mature dendritic cells.

Journal of virology ·Vol. 81 ·No. 17 ·2007-09-00 ·Pages 8933-43

Wang JH, Janas AM, Olson WJ, Wu L

Abstract

Dendritic cells (DCs) potently stimulate the transmission of human immunodeficiency virus type 1 (HIV-1) to CD4(+) T cells. Immature DCs (iDCs) located in submucosal tissues can capture HIV-1 and migrate to lymphoid tissues, where they become mature DCs (mDCs) for effective antigen presentation. DC maturation promotes HIV-1 transmission; however, the underlying mechanisms remain unclear. Here we have compared monocyte-derived iDCs and mDCs for their efficiencies and mechanisms of HIV-1 transmission. We have found that mDCs significantly facilitate HIV-1 endocytosis and efficiently concentrate HIV-1 at virological synapses, which contributes to mDC-enhanced viral transmission, at least in part. mDCs were more efficient than iDCs in transferring HIV-1 to various types of target cells independently of C-type lectins, which partially accounted for iDC-mediated HIV-1 transmission. Efficient HIV-1 trans-infection mediated by iDCs and mDCs required contact between DCs and target cells. Moreover, rapid HIV-1 degradation occurred in both iDCs and mDCs, which correlated with the lack of HIV-1 retention-mediated long-term viral transmission. Our results provide new insights into the mechanisms underlying DC-mediated HIV-1 transmission, suggesting that HIV-1 exploits mDCs to facilitate its dissemination within lymphoid tissues.

MeSH Terms
Cells, Cultured Dendritic Cells/immunology,ultrastructure,virology Endocytosis HIV-1/growth & development,physiology Humans Lectins, C-Type/physiology Microscopy, Electron, Transmission Virus Internalization
Chemicals
Lectins, C-Type
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang Jian-Hua
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Janas Alicia M
Olson Wendy J
Wu Li
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.00878-07
Published
2007-09-00
Epub
2007-00-13
Pages
8933-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC1951429
Subset
IM
Grants
NIAID NIH HHS · R01 AI068493 · United States
NIAID NIH HHS · R01 AI068493-02 · United States
External Links
PubMed source DOI: 10.1128/JVI.00878-07 PMC Full Text View journal details
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