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PMID: 17538020 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their budding.

Molecular biology of the cell ·Vol. 18 ·No. 8 ·2007-08-00 ·Pages 3193-203

Camus G, Segura-Morales C, Molle D, Lopez-Vergès S, Begon-Pescia C, Cazevieille C, Schu P, Bertrand E, Berlioz-Torrent C, Basyuk E

Abstract

Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by genetic knock-out or by RNA interference reduces budding of murine leukemia virus (MLV) and HIV-1, leading to a delay of viral propagation in cell culture. In contrast, overexpression of AP-1mu enhances release of HIV-1 Gag. We show that the AP-1 complex facilitates retroviral budding through a direct interaction between the matrix and AP-1mu. Less MLV Gag is found associated with late endosomes in cells lacking AP-1, and our results suggest that AP-1 and AP-3 could function on the same pathway that leads to Gag release. In addition, we find that AP-1 interacts with Tsg101 and Nedd4.1, two cellular proteins known to be involved in HIV-1 and MLV budding. We propose that AP-1 promotes Gag release by transporting it to intracellular sites of active budding, and/or by facilitating its interactions with other cellular partners.

MeSH Terms
Adaptor Protein Complex 1/metabolism Adaptor Protein Complex 3/metabolism Adaptor Protein Complex mu Subunits/metabolism Animals Carrier Proteins/metabolism DNA-Binding Proteins/metabolism Endosomal Sorting Complexes Required for Transport Gene Products, gag/metabolism HIV-1/physiology,ultrastructure HeLa Cells Humans Leukemia Virus, Murine/physiology Mice Mutation/genetics Protein Binding Protein Transport Rats Transcription Factors/metabolism Two-Hybrid System Techniques Virus Replication
Chemicals
Adaptor Protein Complex 1 Adaptor Protein Complex 3 Adaptor Protein Complex mu Subunits Carrier Proteins DNA-Binding Proteins Endosomal Sorting Complexes Required for Transport Gene Products, gag Transcription Factors Tsg101 protein
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Camus Grégory
Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique (UMR 8104), Paris, France.
Segura-Morales Carolina
Molle Dorothee
Lopez-Vergès Sandra
Begon-Pescia Christina
Cazevieille Chantal
Schu Peter
Bertrand Edouard
Berlioz-Torrent Clarisse
Basyuk Eugenia
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-12-1147
Published
2007-08-00
Epub
2007-00-30
Pages
3193-203
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1949356
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e06-12-1147 PMC Full Text View journal details
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