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PMID: 17526749 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

An SNF2 protein associated with nuclear RNA silencing and the spread of a silencing signal between cells in Arabidopsis.

The Plant cell ·Vol. 19 ·No. 5 ·2007-05-00 ·Pages 1507-21

Smith LM, Pontes O, Searle I, Yelina N, Yousafzai FK, Herr AJ, Pikaard CS, Baulcombe DC

Abstract

The silencing phenotype in Arabidopsis thaliana lines with an inverted repeat transgene under the control of a phloem-specific promoter was manifested in regions around veins due to a mobile signal of silencing. Genetic analysis implicates RNA-DEPENDENT RNA POLYMERASE2 (RDR2) and an RNA polymerase IVa subunit gene (NRPD1a) in the signaling mechanism. We also identified an SNF2 domain-containing protein (CLASSY1) that acts together with RDR2 and NRPD1a in the spread of transgene silencing and in the production of endogenous 24-nucleotide short interfering RNAs (siRNAs). Cytochemical analysis indicates that CLASSY1 may act in the nucleus with NRPD1a and RDR2 in the upstream part of RNA silencing pathways that generate a double-stranded RNA substrate for Dicer-like (DCL) nucleases. DCL3 and ARGONAUTE4 act in a downstream part of the pathway, leading to endogenous 24-nucleotide siRNA production, but are not required for intercellular signaling. From genetic analysis, we conclude that another downstream part of the pathway associated with intercellular signaling requires DCL4 and at least one other protein required for 21-nucleotide trans-acting siRNAs. We interpret the effect of polymerase IVa and trans-acting siRNA pathway mutations in terms of a modular property of RNA silencing pathways.

MeSH Terms
Alleles Amino Acid Sequence Arabidopsis/cytology,enzymology,genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism Cell Nucleus/metabolism DNA Polymerase beta/metabolism Gene Expression Regulation, Plant Genes, Reporter Intracellular Signaling Peptides and Proteins/chemistry,genetics,metabolism MicroRNAs/metabolism Models, Biological Molecular Sequence Data Mutagenesis Mutation/genetics Phenotype Plants, Genetically Modified Protein Structure, Secondary Protein Structure, Tertiary Protein Transport RNA Interference RNA, Small Interfering/metabolism RNA-Dependent RNA Polymerase/metabolism Signal Transduction Subcellular Fractions/metabolism
Chemicals
Arabidopsis Proteins CLASSY1 protein, Arabidopsis Intracellular Signaling Peptides and Proteins MicroRNAs RNA, Small Interfering RDR2 protein, Arabidopsis RNA-Dependent RNA Polymerase DNA Polymerase beta
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Smith Lisa M
Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom.
Pontes Olga
Searle Iain
Yelina Nataliya
Yousafzai Faridoon K
Herr Alan J
Pikaard Craig S
Baulcombe David C
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.051540
Published
2007-05-00
Epub
2007-00-25
Pages
1507-21
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1913737
Subset
IM
Grants
NIGMS NIH HHS · R01 GM077590 · United States
NIGMS NIH HHS · R01GM-077590 · United States
External Links
PubMed source DOI: 10.1105/tpc.107.051540 PMC Full Text View journal details
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