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PMID: 17429573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure-dependent relationships between growth temperature of prokaryotes and the amino acid frequency in their proteins.

Extremophiles : life under extreme conditions ·Vol. 11 ·No. 4 ·2007-07-00 ·Pages 585-96

Saelensminde G, Halskau Ø, Helland R, Willassen NP, Jonassen I

Abstract

We studied the amino acid frequency and substitution patterns between homologues of prokaryotic species adapted to temperatures in the range 0-102 degrees C, and found a significant temperature-dependent difference in frequency for many of the amino acids. This was particularly clear when we analysed the surface and core residues separately. The difference between the surface and the core is getting more pronounced in proteins adapted to warmer environments, with a more hydrophobic core, and more charged and long-chained amino acids on the surface of the proteins. We also see that mesophiles have a more similar amino acid composition to psychrophiles than to thermophiles, and that archea appears to have a slightly different pattern of substitutions than bacteria.

MeSH Terms
Adaptation, Physiological Amino Acid Sequence Amino Acid Substitution Amino Acids/chemistry Archaea/growth & development,metabolism Archaeal Proteins/chemistry,metabolism Bacteria/growth & development,metabolism Bacterial Proteins/chemistry,metabolism Databases, Protein Prokaryotic Cells Protein Conformation Protein Denaturation Sequence Alignment Sequence Analysis, Protein Structure-Activity Relationship Surface Properties Temperature
Chemicals
Amino Acids Archaeal Proteins Bacterial Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Saelensminde Gisle
Computational Biology Unit (CBU), BCCS, University of Bergen, Bergen, Norway. gisle@cbu.uib.no
Halskau Øyvind
Helland Ronny
Willassen Nils-Peder
Jonassen Inge
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Article Info
Journal
Extremophiles : life under extreme conditions
Abbr.
Extremophiles
ISSN
1431-0651
DOI
10.1007/s00792-007-0072-3
Email
gisle@cbu.uib.no
Published
2007-07-00
Epub
2007-00-12
Pages
585-96
Language
English
Region
Germany
NLM ID
9706854
Subset
IM
External Links
PubMed source DOI: 10.1007/s00792-007-0072-3 View journal details
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