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PMID: 1740467 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a subdomain of CENP-B that is necessary and sufficient for localization to the human centromere.

The Journal of cell biology ·Vol. 116 ·No. 5 ·1992-03-00 ·Pages 1081-93

Pluta AF, Saitoh N, Goldberg I, Earnshaw WC

Abstract

We have combined in vivo and in vitro approaches to investigate the function of CENP-B, a major protein of human centromeric heterochromatin. Expression of epitope-tagged deletion derivatives of CENP-B in HeLa cells revealed that a single domain less than 158 residues from the amino terminus of the protein is sufficient to localize CENP-B to centromeres. Centromere localization was abolished if as few as 28 amino acids were removed from the amino terminus of CENP-B. The centromere localization signal of CENP-B can function in an autonomous fashion, relocating a fused bacterial enzyme to centromeres. The centromere localization domain of CENP-B specifically binds in vitro to a subset of alpha-satellite DNA monomers. These results suggest that the primary mechanism for localization of CENP-B to centromeres involves the recognition of a DNA sequence found at centromeres. Analysis of the distribution of this sequence in alpha-satellite DNA suggests that CENP-B binding may have profound effects on chromatin structure at centromeres.

MeSH Terms
Autoantigens Base Sequence Centromere/chemistry Centromere Protein B Chromatin/chemistry Chromosomal Proteins, Non-Histone/analysis DNA-Binding Proteins/analysis Gene Expression Regulation HeLa Cells Humans Models, Molecular Molecular Sequence Data Protein Conformation
Chemicals
Autoantigens CENPB protein, human Centromere Protein B Chromatin Chromosomal Proteins, Non-Histone DNA-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pluta A F
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Saitoh N
Goldberg I
Earnshaw W C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.116.5.1081
Published
1992-03-00
Pages
1081-93
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289363
Subset
IM
Grants
NIGMS NIH HHS · GM 35212 · United States
External Links
PubMed source DOI: 10.1083/jcb.116.5.1081 PMC Full Text View journal details
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