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PMID: 1736898 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein.

The Biochemical journal ·Vol. 281 ( Pt 2) ·1992-01-15 ·Pages 513-7

Damjanovski S, Liu F, Ringuette M

Abstract

SPARC (Secreted Protein, Acidic, Rich in Cysteine) is expressed as a 1.6 kb mRNA in Xenopus laevis. On the basis of cDNA sequence analysis, Xenopus SPARC has a core Mr of 32643, with one potential N-glycosylation site. Western analysis of SPARC isolated from Xenopus long bone indicates that the mature protein has an Mr of 43,000. At the amino acid level, Xenopus SPARC has 78-79% sequence similarity to mouse, bovine and human SPARC. The least-conserved region is found within the N-terminal glutamic acid-rich domain, with the C-terminal Ca(2+)-binding domain being the most conserved. Adult Xenopus tissues show the same pattern of tissue-specific distribution of SPARC mRNAs as adult mouse.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Biological Evolution Blotting, Western DNA Extracellular Matrix Proteins/chemistry,genetics Female Gene Expression Hydrogen-Ion Concentration Male Mice Molecular Sequence Data Organ Specificity/genetics Osteonectin/chemistry,genetics Protein Conformation Xenopus laevis

Chemicals

Extracellular Matrix Proteins Osteonectin DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Damjanovski S

Department of Zoology, University of Toronto, Ontario, Canada.

Liu F

Ringuette M

References (23)

23 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2810513

Published

1992-01-15

Pages

513-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1130715

Subset

Databases

GENBANK

M57479, M86474, M86475, M86476, M86477, M86478, M86479, M86480, M86481, X62483

External Links

PubMed source DOI: 10.1042/bj2810513 PMC Full Text View journal details

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Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein.

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