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PMID: 17360778 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tombola, a tesmin/TSO1-family protein, regulates transcriptional activation in the Drosophila male germline and physically interacts with always early.

Development (Cambridge, England) ·Vol. 134 ·No. 8 ·2007-04-00 ·Pages 1549-59

Jiang J, Benson E, Bausek N, Doggett K, White-Cooper H

Abstract

During male gametogenesis, a developmentally regulated and cell type-specific transcriptional programme is activated in primary spermatocytes to prepare for differentiation of sperm. The Drosophila aly-class meiotic-arrest loci (aly, comr, achi/vis and topi) are essential for activation of transcription of many differentiation-specific genes, and several genes important for meiotic cell cycle progression, thus linking meiotic divisions to cellular differentiation during spermatogenesis. Protein interaction studies suggest that the aly-class gene products form a chromatin-associated complex in primary spermatocytes. We identify, clone and characterise a new aly-class meiotic-arrest gene, tombola (tomb), which encodes a testis-specific CXC-domain protein that interacts with Aly. The tomb mutant phenotype is more like that of aly and comr mutants than that of achi/vis or topi mutants in terms of target gene profile and chromosome morphology. tomb encodes a chromatin-associated protein required for localisation of Aly and Comr, but not Topi, to chromatin Reciprocally, aly and comr, but not topi or achi/vis, are required to maintain the normal localisation of Tomb. tomb and aly might be components of a complex paralogous to the Drosophila dREAM/Myb-MuvB and C. elegans DRM transcriptional regulatory complexes.

MeSH Terms
Amino Acid Sequence Animals Cell Cycle Proteins/genetics,metabolism Cell Differentiation Cell Nucleus/metabolism Drosophila/genetics,metabolism Drosophila Proteins/genetics,metabolism Male Molecular Sequence Data Mutation Nuclear Proteins/metabolism Protein Binding Spermatocytes/metabolism,physiology Transcriptional Activation
Chemicals
Cell Cycle Proteins Drosophila Proteins Nuclear Proteins aly protein, Drosophila comr protein, Drosophila tomb protein, Drosophila
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jiang Jianqiao
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
Benson Elizabeth
Bausek Nina
Doggett Karen
White-Cooper Helen
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Article Info
Journal
Development (Cambridge, England)
Abbr.
Development
ISSN
0950-1991
DOI
10.1242/dev.000521
Published
2007-04-00
Epub
2007-00-14
Pages
1549-59
Language
English
Region
England
NLM ID
8701744
PMCID
PMC2229809
Subset
IM
Grants
Wellcome Trust · 073930 · United Kingdom
Wellcome Trust · 075852 · United Kingdom
External Links
PubMed source DOI: 10.1242/dev.000521 PMC Full Text View journal details
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