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PMID: 16624904 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Identification and classification of genes that act antagonistically to let-60 Ras signaling in Caenorhabditis elegans vulval development.

Genetics ·Vol. 173 ·No. 2 ·2006-06-00 ·Pages 709-26

Ceol CJ, Stegmeier F, Harrison MM, Horvitz HR

Abstract

The synthetic multivulva (synMuv) genes negatively regulate Ras-mediated vulval induction in the nematode Caenorhabditis elegans. The synMuv genes define three classes, A, B, and C, such that double mutants carrying mutations in genes of any two classes are multivulva. The class B synMuv genes include lin-35, a homolog of the retinoblastoma (Rb) tumor suppressor gene, as well as homologs of genes that function with Rb in transcriptional regulation. We screened for additional synMuv mutations using a strategy different from that of previous synMuv genetic screens. Some of the mutations we recovered affect new synMuv genes. We present criteria for assigning synMuv mutations into different genetic classes. We also describe the molecular characterization of the class B synMuv gene lin-65.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans/genetics,growth & development,metabolism Caenorhabditis elegans Proteins/genetics,metabolism Crosses, Genetic DNA, Helminth/genetics Female Genes, Helminth Genetic Linkage Heterozygote Male Molecular Sequence Data Mutation Phenotype Signal Transduction Vulva/growth & development ras Proteins/genetics,metabolism
Chemicals
Caenorhabditis elegans Proteins DNA, Helminth let-60 protein, C elegans ras Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ceol Craig J
Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Stegmeier Frank
Harrison Melissa M
Horvitz H Robert
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.106.056465
Published
2006-06-00
Epub
2006-00-19
Pages
709-26
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1526536
Subset
IM
Grants
NIGMS NIH HHS · R01 GM024663 · United States
NIGMS NIH HHS · R37 GM024663 · United States
NIGMS NIH HHS · GM24663 · United States
Databases
GENBANK
AY818712, AY818713
External Links
PubMed source DOI: 10.1534/genetics.106.056465 PMC Full Text View journal details
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