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PMID: 17360450
Published · ppublish
English
Journal Article
Research Support, Non-U.S. Gov't
Prospects for fungus identification using CO1 DNA barcodes, with Penicillium as a test case.
Proceedings of the National Academy of Sciences of the United States of America
·Vol. 104
·No. 10
·2007-03-06
·Pages 3901-6
Abstract
DNA barcoding systems employ a short, standardized gene region to identify species. A 648-bp segment of mitochondrial cytochrome c oxidase 1 (CO1) is the core barcode region for animals, but its utility has not been tested in fungi. This study began with an examination of patterns of sequence divergences in this gene region for 38 fungal taxa with full CO1 sequences. Because these results suggested that CO1 could be effective in species recognition, we designed primers for a 545-bp fragment of CO1 and generated sequences for multiple strains from 58 species of Penicillium subgenus Penicillium and 12 allied species. Despite the frequent literature reports of introns in fungal mitochondrial genomes, we detected introns in only 2 of 370 Penicillium strains. Representatives from 38 of 58 species formed cohesive assemblages with distinct CO1 sequences, and all cases of sequence sharing involved known species complexes. CO1 sequence divergences averaged 0.06% within species, less than for internal transcribed spacer nrDNA or beta-tubulin sequences (BenA). CO1 divergences between species averaged 5.6%, comparable to internal transcribed spacer, but less than values for BenA (14.4%). Although the latter gene delivered higher taxonomic resolution, the amplification and alignment of CO1 was simpler. The development of a barcoding system for fungi that shares a common gene target with other kingdoms would be a significant advance.
MeSH Terms
DNA/genetics
DNA Primers/chemistry,genetics
DNA, Fungal/genetics
DNA, Intergenic
Electron Transport Complex IV/genetics
Genes, Fungal
Genetic Techniques
Genetic Variation
Introns
Molecular Sequence Data
Penicillium/genetics
Phylogeny
Research Design
Tubulin/genetics
Chemicals
DNA Primers
DNA, Fungal
DNA, Intergenic
Tubulin
DNA
Electron Transport Complex IV
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Email
Published
2007-03-06
Epub
2007-00-28
Pages
3901-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1805696
Subset
IM
Databases
GENBANK
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