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PMID: 17353356 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Requirement of ZO-1 for the formation of belt-like adherens junctions during epithelial cell polarization.

The Journal of cell biology ·Vol. 176 ·No. 6 ·2007-03-12 ·Pages 779-86

Ikenouchi J, Umeda K, Tsukita S, Furuse M, Tsukita S

Abstract

The molecular mechanisms of how primordial adherens junctions (AJs) evolve into spatially separated belt-like AJs and tight junctions (TJs) during epithelial polarization are not well understood. Previously, we reported the establishment of ZO-1/ZO-2-deficient cultured epithelial cells (1[ko]/2[kd] cells), which lacked TJs completely. In the present study, we found that the formation of belt-like AJs was significantly delayed in 1(ko)/2(kd) cells during epithelial polarization. The activation of Rac1 upon primordial AJ formation is severely impaired in 1(ko)/2(kd) cells. Our data indicate that ZO-1 plays crucial roles not only in TJ formation, but also in the conversion from "fibroblastic" AJs to belt-like "polarized epithelial" AJs through Rac1 activation. Furthermore, to examine whether ZO-1 itself mediate belt-like AJ and TJ formation, respectively, we performed a mutational analysis of ZO-1. The requirement for ZO-1 differs between belt-like AJ and TJ formation. We propose that ZO-1 is directly involved in the establishment of two distinct junctional domains, belt-like AJs and TJs, during epithelial polarization.

MeSH Terms
Actin Cytoskeleton/metabolism,ultrastructure Adherens Junctions/metabolism,ultrastructure Animals Cell Line Cell Polarity/physiology Epithelial Cells/cytology,ultrastructure Membrane Proteins/antagonists & inhibitors,genetics,physiology Mice Neuropeptides/metabolism Phosphoproteins/antagonists & inhibitors,genetics,physiology Tight Junctions/metabolism Zonula Occludens-1 Protein Zonula Occludens-2 Protein rac GTP-Binding Proteins/metabolism rac1 GTP-Binding Protein
Chemicals
Membrane Proteins Neuropeptides Phosphoproteins Rac1 protein, mouse Tjp1 protein, mouse Tjp2 protein, mouse Zonula Occludens-1 Protein Zonula Occludens-2 Protein rac GTP-Binding Proteins rac1 GTP-Binding Protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ikenouchi Junichi
Department of Cell Biology, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. ikenouti@mfour.med.kyoto-u.ac.jp
Umeda Kazuaki
Tsukita Sachiko
Furuse Mikio
Tsukita Shoichiro
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200612080
Email
ikenouti@mfour.med.kyoto-u.ac.jp
Published
2007-03-12
Pages
779-86
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064052
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200612080 PMC Full Text View journal details
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