Home LiteratureArticle Details
PMID: 17351089 Published · ppublish English Comparative Study Journal Article

Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro.

Applied and environmental microbiology ·Vol. 73 ·No. 9 ·2007-05-00 ·Pages 3091-4

Riedel CU, Monk IR, Casey PG, Morrissey D, O'Sullivan GC, Tangney M, Hill C, Gahan CG

Abstract

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

MeSH Terms
Animals Base Sequence Food Inspection/methods Food Microbiology Genetic Vectors/genetics Listeria monocytogenes/metabolism Luciferases/metabolism Luciferases, Bacterial Mice Molecular Sequence Data Operon/genetics Promoter Regions, Genetic/genetics Sequence Analysis, DNA
Chemicals
Luciferases Luciferases, Bacterial
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Riedel Christian U
Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland.
Monk Ian R
Casey Pat G
Morrissey David
O'Sullivan Gerald C
Tangney Mark
Hill Colin
Gahan Cormac G M
References (27)
27 references, click to expand
  1. Monitoring bioluminescent Staphylococcus aureus infections in living mice using a novel luxABCDE construct.
    Infect Immun. 2000 Jun;68(6):3594-600 PMID: 10816517
  2. In vivo effects of sporulation kinases on mutant Spo0A proteins in Bacillus subtilis.
    J Bacteriol. 2001 Nov;183(22):6573-8 PMID: 11673427
  3. Implication of milk and milk products in food-borne diseases in France and in different industrialised countries.
    Int J Food Microbiol. 2001 Jul 20;67(1-2):1-17 PMID: 11482557
  4. Comparative genomics of Listeria species.
    Science. 2001 Oct 26;294(5543):849-52 PMID: 11679669
  5. pfs-dependent regulation of autoinducer 2 production in Salmonella enterica serovar Typhimurium.
    J Bacteriol. 2002 Jul;184(13):3450-6 PMID: 12057938
  6. Construction, characterization, and use of two Listeria monocytogenes site-specific phage integration vectors.
    J Bacteriol. 2002 Aug;184(15):4177-86 PMID: 12107135
  7. Bactofection of mammalian cells by Listeria monocytogenes: improvement and mechanism of DNA delivery.
    Gene Ther. 2003 Nov;10(24):2036-45 PMID: 14566363
  8. Extracellular replication of Listeria monocytogenes in the murine gall bladder.
    Science. 2004 Feb 6;303(5659):851-3 PMID: 14764883
  9. Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins.
    Nat Biotechnol. 2004 Mar;22(3):313-20 PMID: 14990953
  10. Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species.
    Nucleic Acids Res. 2004;32(8):2386-95 PMID: 15115801
  11. Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence.
    Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12318-23 PMID: 15302931
  12. Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusion and an E. coli strain carrying a grpE'::lux fusion are similar.
    Appl Environ Microbiol. 1995 Nov;61(11):4124-7 PMID: 8526529
  13. Oxidative stress detection with Escherichia coli harboring a katG'::lux fusion.
    Appl Environ Microbiol. 1996 Jul;62(7):2252-6 PMID: 8779563
  14. Photonic detection of bacterial pathogens in living hosts.
    Mol Microbiol. 1995 Nov;18(4):593-603 PMID: 8817482
  15. Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.
    Appl Environ Microbiol. 1997 Jul;63(7):2566-71 PMID: 9212407
  16. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters.
    Appl Environ Microbiol. 1998 Jan;64(1):82-7 PMID: 9435063
  17. Real-time monitoring of Escherichia coli O157:H7 adherence to beef carcass surface tissues with a bioluminescent reporter.
    Appl Environ Microbiol. 1999 Apr;65(4):1738-45 PMID: 10103275
  18. Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster.
    Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15573-8 PMID: 15496466
  19. Monitoring age-related susceptibility of young mice to oral Salmonella enterica serovar Typhimurium infection using an in vivo murine model.
    Pediatr Res. 2005 Jul;58(1):153-8 PMID: 15774831
  20. The 5' untranslated region-mediated enhancement of intracellular listeriolysin O production is required for Listeria monocytogenes pathogenicity.
    Mol Microbiol. 2005 Sep;57(5):1460-73 PMID: 16102013
  21. Citrobacter rodentium of mice and man.
    Cell Microbiol. 2005 Dec;7(12):1697-706 PMID: 16309456
  22. Bacterial gene therapy strategies.
    J Pathol. 2006 Jan;208(2):290-8 PMID: 16362987
  23. Novel luciferase reporter system for in vitro and organ-specific monitoring of differential gene expression in Listeria monocytogenes.
    Appl Environ Microbiol. 2006 Apr;72(4):2876-84 PMID: 16597994
  24. An updated review of Listeria monocytogenes in the pork meat industry and its products.
    J Appl Microbiol. 2006 Jul;101(1):7-17 PMID: 16834586
  25. Local gene therapy of solid tumors with GM-CSF and B7-1 eradicates both treated and distal tumors.
    Cancer Gene Ther. 2006 Dec;13(12):1061-71 PMID: 16874363
  26. agr expression precedes escape of internalized Staphylococcus aureus from the host endosome.
    Infect Immun. 2001 Nov;69(11):7074-82 PMID: 11598083
  27. Visualizing pneumococcal infections in the lungs of live mice using bioluminescent Streptococcus pneumoniae transformed with a novel gram-positive lux transposon.
    Infect Immun. 2001 May;69(5):3350-8 PMID: 11292758
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.02940-06
Published
2007-05-00
Epub
2007-00-09
Pages
3091-4
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1892880
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.02940-06 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2