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PMID: 11673427 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vivo effects of sporulation kinases on mutant Spo0A proteins in Bacillus subtilis.

Journal of bacteriology ·Vol. 183 ·No. 22 ·2001-11-00 ·Pages 6573-8

Quisel JD, Burkholder WF, Grossman AD

Abstract

The phosphorylated form of the response regulator Spo0A (Spo0A~P) is required for the initiation of sporulation in Bacillus subtilis. Phosphate is transferred to Spo0A from at least four histidine kinases (KinA, KinB, KinC, and KinD) by a phosphotransfer pathway composed of Spo0F and Spo0B. Several mutations in spo0A allow initiation of sporulation in the absence of spo0F and spo0B, but the mechanisms by which these mutations allow bypass of spo0F and spo0B are not fully understood. We measured the ability of KinA, KinB, and KinC to activate sporulation of five spo0A mutants in the absence of Spo0F and Spo0B. We also determined the effect of Spo0E, a Spo0A~P-specific phosphatase, on sporulation of strains containing the spo0A mutations. Our results indicate that several of the mutations relax the specificity of Spo0A, allowing Spo0A to obtain phosphate from a broader group of phosphodonors. In the course of these experiments, we observed medium-dependent effects on the sporulation of different mutants. This led us to identify a small molecule, acetoin, that can stimulate sporulation of some spo0A mutants.

MeSH Terms
Acetoin/pharmacology Bacillus subtilis/drug effects,genetics Bacterial Proteins/genetics,metabolism Histidine Kinase Mutation Phosphotransferases Protein Kinases/metabolism,physiology Sigma Factor Signal Transduction Spores, Bacterial Transcription Factors/genetics
Chemicals
Bacterial Proteins Sigma Factor Spo0A protein, Bacillus subtilis Spo0F protein, Bacillus subtilis Transcription Factors kinA protein, Bacillus subtilis spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus Acetoin Phosphotransferases Protein Kinases Histidine Kinase kinB protein, Bacillus subtilis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Quisel J D
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Burkholder W F
Grossman A D
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.22.6573-6578.2001
Published
2001-11-00
Pages
6573-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95488
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041934 · United States
NIGMS NIH HHS · R37 GM041934 · United States
NIGMS NIH HHS · GM41934 · United States
External Links
PubMed source DOI: 10.1128/JB.183.22.6573-6578.2001 PMC Full Text View journal details
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