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PMID: 17339418 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Friend of GATA-1-independent transcriptional repression: a novel mode of GATA-1 function.

Blood ·Vol. 109 ·No. 12 ·2007-06-15 ·Pages 5230-3

Johnson KD, Boyer ME, Kang JA, Wickrema A, Cantor AB, Bresnick EH

Abstract

The GATA-1-interacting protein Friend Of GATA-1 (FOG-1) is essential for the proper transcriptional activation and repression of numerous GATA-1 target genes. Although FOG-1-independent activation by GATA-1 has been described, all known examples of GATA-1-mediated repression are FOG-1 dependent. In the GATA-1-null G1E cell line, estrogen receptor ligand binding domain (ER) chimeras of either wild-type GATA-1 or a FOG-1-binding defective mutant of GATA-1 repressed several genes similarly upon activation with beta-estradiol. Repression also occurred in a FOG-1-null cell line expressing ER-GATA-1 and during ex vivo erythropoiesis. At the Lyl1 and Rgs18 loci, we found highly restricted occupancy by GATA-1 and GATA-2, indicating that these genes are direct targets of GATA factor regulation. The identification of genes repressed by GATA-1 independent of FOG-1 defines a novel mode of GATA-1-mediated transcriptional regulation.

MeSH Terms
Animals Binding Sites GATA1 Transcription Factor/genetics,physiology Gene Expression Regulation Mice Mutation Nuclear Proteins/genetics Receptors, Estrogen/genetics Recombinant Fusion Proteins Transcription Factors/genetics Transcription, Genetic
Chemicals
GATA1 Transcription Factor Gata1 protein, mouse Nuclear Proteins Receptors, Estrogen Recombinant Fusion Proteins Transcription Factors Zfpm1 protein, mouse
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Johnson Kirby D
Department of Pharmacology, University of Wisconsin School of Medicine, Madison, WI, USA.
Boyer Meghan E
Kang Jeong-Ah
Wickrema Amittha
Cantor Alan B
Bresnick Emery H
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
0006-4971
DOI
10.1182/blood-2007-02-072983
Published
2007-06-15
Epub
2007-00-05
Pages
5230-3
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC1890840
Subset
IM
Grants
NIDDK NIH HHS · K01 DK069488 · United States
NIDDK NIH HHS · DK68634 · United States
NIDDK NIH HHS · DK55700 · United States
NIDDK NIH HHS · DK069488 · United States
NIDDK NIH HHS · R01 DK055700 · United States
NIDDK NIH HHS · R01 DK068634 · United States
NIDDK NIH HHS · R56 DK068634 · United States
External Links
PubMed source DOI: 10.1182/blood-2007-02-072983 PMC Full Text View journal details
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