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PMID: 1732730 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The restricted promoter activity of the liver transcription factor hepatocyte nuclear factor 3 beta involves a cell-specific factor and positive autoactivation.

Molecular and cellular biology ·Vol. 12 ·No. 2 ·1992-02-00 ·Pages 552-62

Pani L, Quian XB, Clevidence D, Costa RH

Abstract

The transcription factor hepatocyte nuclear factor 3 (HNF-3) is involved in the coordinate expression of several liver genes. HNF-3 DNA binding activity is composed of three different liver proteins which recognize the same DNA site. The HNF-3 proteins (designated alpha, beta, and gamma) possess homology in the DNA binding domain and in several additional regions. To understand the cell-type-specific expression of HNF-3 beta, we have defined the regulatory sequences that elicit hepatoma-specific expression. Promoter activity requires -134 bp of HNF-3 beta proximal sequences and binds four nuclear proteins, including two ubiquitous factors. One of these promoter sites interacts with a novel cell-specific factor, LF-H3 beta, whose binding activity correlates with the HNF-3 beta tissue expression pattern. Furthermore, there is a binding site for the HNF-3 protein within its own promoter, suggesting that an autoactivation mechanism is involved in the establishment of HNF-3 beta expression. We propose that both the LF-H3 beta and HNF-3 sites play an important role in the cell-type-specific expression of the HNF-3 beta transcription factor.

MeSH Terms
Base Sequence Binding Sites/genetics Cloning, Molecular DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation/physiology Hepatocyte Nuclear Factor 3-beta Humans Liver/metabolism Methylation Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins/genetics,metabolism Promoter Regions, Genetic/physiology Transcription Factors/genetics,metabolism Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins FOXA2 protein, human Nuclear Proteins Transcription Factors Hepatocyte Nuclear Factor 3-beta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pani L
Department of Biochemistry, University of Illinois College of Medicine, Chicago 60612.
Quian X B
Clevidence D
Costa R H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.2.552-562.1992
Published
1992-02-00
Pages
552-62
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364229
Subset
IM
Grants
NIGMS NIH HHS · GM43241 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.2.552-562.1992 PMC Full Text View journal details
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