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PMID: 17321604 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: new insights into blood-brain barrier dysfunction for HIV-1-associated dementia.

Journal of neuroimmunology ·Vol. 185 ·No. 1-2 ·2007-04-00 ·Pages 37-46

Ricardo-Dukelow M, Kadiu I, Rozek W, Schlautman J, Persidsky Y, Ciborowski P, Kanmogne GD, Gendelman HE

Abstract

Blood-brain barrier (BBB) compromise and transendothelial migration of HIV-infected leukocytes into the central nervous system (CNS) underlies the neuropathogenesis of HIV-1 infection. How this occurs is incompletely understood. We used a proteomic platform integrating difference gel electrophoresis and tandem mass spectrometry peptide sequencing to determine the effects that HIV-1-infected macrophages have on human brain microvascular endothelial cell (HBMEC) protein profiles. HIV-1 infected monocyte-derived macrophages (MDM) induced the upregulation of over 200 HBMEC proteins. These included metabolic, voltage-gated ion channels, heat shock, transport, cytoskeletal, regulatory, and calcium binding proteins. Results were validated by Western blot analysis. We conclude that HIV-1-infected MDM affect the HBMEC proteome and, in this way, affect BBB dysfunction and the development of HIV-1 CNS disease.

MeSH Terms
AIDS Dementia Complex/physiopathology Blood-Brain Barrier/physiology,virology Blotting, Western Brain/virology Cells, Cultured Coculture Techniques Electrophoresis, Gel, Two-Dimensional Endothelial Cells/physiology Gene Expression Gene Expression Regulation HIV Infections/physiopathology HIV-1 Humans Image Processing, Computer-Assisted Macrophages/virology Proteomics Tandem Mass Spectrometry
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ricardo-Dukelow Mary
Center for Neurovirology and Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198-5215, USA.
Kadiu Irena
Rozek Wojciech
Schlautman Joshua
Persidsky Yuri
Ciborowski Pawel
Kanmogne Georgette D
Gendelman Howard E
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Article Info
Journal
Journal of neuroimmunology
Abbr.
J Neuroimmunol
ISSN
0165-5728
DOI
10.1016/j.jneuroim.2007.01.004
Published
2007-04-00
Epub
2007-00-23
Pages
37-46
Language
English
Region
Netherlands
NLM ID
8109498
PMCID
PMC2212599
Subset
IM
Grants
NINDS NIH HHS · T32 NS007488-05 · United States
NINDS NIH HHS · R01 NS036126 · United States
NIAAA NIH HHS · R01 AA015913-02 · United States
NCRR NIH HHS · P20 RR15635-03 · United States
NIMH NIH HHS · K01 MH068214-05 · United States
NINDS NIH HHS · P01 NS043985-01A1 · United States
NINDS NIH HHS · P01 NS043985 · United States
NIMH NIH HHS · R01 MH065151 · United States
NIMH NIH HHS · MH068214 · United States
NIAAA NIH HHS · R21 AA013846-03 · United States
NIMH NIH HHS · K01 MH068214 · United States
NINDS NIH HHS · 1T32 NS07488 · United States
NIMH NIH HHS · P01 MH064570-03 · United States
NIAAA NIH HHS · R37 AA015913 · United States
NIAAA NIH HHS · R21 AA013846 · United States
NIMH NIH HHS · P01 MH064570 · United States
NIMH NIH HHS · 5 P01 MH64570-03 · United States
NINDS NIH HHS · R37 NS036126 · United States
NINDS NIH HHS · T32 NS007488 · United States
NCRR NIH HHS · P20 RR015635 · United States
NINDS NIH HHS · R37 NS036126-10 · United States
NIAAA NIH HHS · R01 AA015913 · United States
NINDS NIH HHS · 2R37 NS36126 · United States
NCRR NIH HHS · P20 RR015635-03 · United States
NINDS NIH HHS · 1 P01 NS043985 · United States
External Links
PubMed source DOI: 10.1016/j.jneuroim.2007.01.004 PMC Full Text View journal details
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