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PMID: 15545357 Published · ppublish English Comparative Study Journal Article

Secretory leukocyte protease inhibitor binds to annexin II, a cofactor for macrophage HIV-1 infection.

The Journal of experimental medicine ·Vol. 200 ·No. 10 ·2004-11-15 ·Pages 1337-46

Ma G, Greenwell-Wild T, Lei K, Jin W, Swisher J, Hardegen N, Wild CT, Wahl SM

Abstract

The distribution of secretory leukocyte protease inhibitor (SLPI) at entry portals indicates its involvement in defending the host from pathogens, consistent with the ability of SLPI to inhibit human immunodeficiency virus (HIV)-1 infection by an unknown mechanism. We now demonstrate that SLPI binds to the membrane of human macrophages through the phospholipid-binding protein, annexin II. Based on the recent identification of human cell membrane phosphatidylserine (PS) in the outer coat of HIV-1, we define a novel role for annexin II, a PS-binding moiety, as a cellular cofactor supporting macrophage HIV-1 infection. Moreover, this HIV-1 PS interaction with annexin II can be disrupted by SLPI or other annexin II-specific inhibitors. The PS-annexin II connection may represent a new target to prevent HIV-1 infection.

MeSH Terms
Annexin A2/metabolism Blotting, Western Cell Line Chromatography, High Pressure Liquid DNA Primers Flow Cytometry HIV Infections/metabolism HIV-1/metabolism Humans Immunoprecipitation Macrophages/metabolism,virology Mass Spectrometry Microscopy, Fluorescence Phosphatidylserines/metabolism Proteinase Inhibitory Proteins, Secretory Proteins/metabolism RNA, Small Interfering/genetics Reverse Transcriptase Polymerase Chain Reaction Secretory Leukocyte Peptidase Inhibitor
Chemicals
Annexin A2 DNA Primers Phosphatidylserines Proteinase Inhibitory Proteins, Secretory Proteins RNA, Small Interfering SLPI protein, human Secretory Leukocyte Peptidase Inhibitor
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ma Ge
Oral Infection and Immunity Branch, NIDCR, NIH, 30 Convent Dr., MSC4352, Building 30, Room 320, Bethesda, MD 20892, USA.
Greenwell-Wild Teresa
Lei Kejian
Jin Wenwen
Swisher Jennifer
Hardegen Neil
Wild Carl T
Wahl Sharon M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20041115
Published
2004-11-15
Pages
1337-46
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2211913
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.20041115 PMC Full Text View journal details
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