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PMID: 1730750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly and disassembly of the Golgi complex: two processes arranged in a cis-trans direction.

The Journal of cell biology ·Vol. 116 ·No. 1 ·1992-01-00 ·Pages 69-83

Alcalde J, Bonay P, Roa A, Vilaro S, Sandoval IV

Abstract

We have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMPc-1, GMPc-2, MG 160) and two trans- (GMPt-1 and GMPt-2) Golgi membrane proteins during and after treatment of normal rat kidney (NRK) cells with brefeldin A (BFA). BFA induced complete disassembly of the cis/middle- and trans-Golgi complex and translocation of GMPc and GMPt to the ER. Cells treated for short times (3 min) with BFA showed extensive disorganization of both cis/middle- and trans-Golgi complexes. However, complete disorganization of the trans part required much longer incubations with the drug. Upon removal of BFA the Golgi complex was reassembled by a process consisting of three steps: (a) exist of cis/middle proteins from the ER and their accumulation into vesicular structures scattered throughout the cytoplasm; (b) gradual relocation and accumulation of the trans proteins in the vesicles containing the cis/middle proteins; and (c) assembly of the cisternae, and reconstruction of the Golgi complex within an area located in the vicinity of the centrosome from which the ER was excluded. Reconstruction of the cis/middle-Golgi complex occurred under temperature conditions inhibitory of the reorganization of the trans-Golgi complex, and was dependent on microtubules. Reconstruction of the trans-Golgi complex, disrupted with nocodazole after selective fusion of the cis/middle-Golgi complex with the ER, occurred after the release of cis/middle-Golgi proteins from the ER and the assembly of the cis/middle cisternae.

MeSH Terms
Animals Anti-Bacterial Agents/pharmacology Brefeldin A Cells, Cultured Cyclopentanes/pharmacology Endoplasmic Reticulum/drug effects,ultrastructure Ethylmaleimide/pharmacology Golgi Apparatus/drug effects,physiology,ultrastructure Kidney Membrane Proteins/analysis,metabolism Microtubules/physiology,ultrastructure Rats Temperature
Chemicals
Anti-Bacterial Agents Cyclopentanes Membrane Proteins Brefeldin A Ethylmaleimide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Alcalde J
Centro de Biologia Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Bonay P
Roa A
Vilaro S
Sandoval I V
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.116.1.69
Published
1992-01-00
Pages
69-83
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289264
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.116.1.69 PMC Full Text View journal details
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