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PMID: 17293426 Published · ppublish English Journal Article

The DeoR-type regulator SugR represses expression of ptsG in Corynebacterium glutamicum.

Journal of bacteriology ·Vol. 189 ·No. 8 ·2007-04-00 ·Pages 2955-66

Engels V, Wendisch VF

Abstract

Corynebacterium glutamicum grows on a variety of carbohydrates and organic acids. Uptake of the preferred carbon source glucose via the phosphoenolpyruvate-dependent phosphotransferase system (PTS) is reduced during coutilization of glucose with acetate, sucrose, or fructose compared to growth on glucose as the sole carbon source. Here we show that the DeoR-type regulator SugR (NCgl1856) represses expression of ptsG, which encodes the glucose-specific PTS enzyme II. Overexpression of sugR resulted in reduced ptsG mRNA levels, decreased glucose utilization, and perturbed growth on media containing glucose. In mutants lacking sugR, expression of the ptsG'-'cat fusion was increased two- to sevenfold during growth on gluconeogenic carbon sources but remained similar during growth on glucose or other sugars. As shown by DNA microarray analysis, SugR also regulates expression of other genes, including ptsS and the putative NCgl1859-fruK-ptsF operon. Purified SugR bound to DNA regions upstream of ptsG, ptsS, and NCgl1859, and a 75-bp ptsG promoter fragment was sufficient for SugR binding. Fructose-6-phosphate interfered with binding of SugR to the ptsG promoter DNA. Thus, while during growth on gluconeogenic carbon sources SugR represses ptsG, ptsG expression is derepressed during growth on glucose or under other conditions characterized by high fructose-6-phosphate concentrations, representing one mechanism which allows C. glutamicum to adapt glucose uptake to carbon source availability.

MeSH Terms
Bacterial Proteins/genetics,physiology Base Sequence Corynebacterium glutamicum/genetics,growth & development Culture Media DNA, Bacterial/metabolism DNA, Intergenic/genetics DNA-Binding Proteins/physiology Down-Regulation Fructosephosphates/metabolism Genes, Bacterial Glucose Molecular Sequence Data Phosphoenolpyruvate Sugar Phosphotransferase System/genetics,metabolism Promoter Regions, Genetic Repressor Proteins/physiology
Chemicals
Bacterial Proteins Culture Media DNA, Bacterial DNA, Intergenic DNA-Binding Proteins Fructosephosphates Repressor Proteins fructose-6-phosphate Phosphoenolpyruvate Sugar Phosphotransferase System Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Engels Verena
Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Corrensstr. 3, D-48149 Muenster, Germany. wendisch@uni-muenster.de
Wendisch Volker F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.01596-06
Email
wendisch@uni-muenster.de
Published
2007-04-00
Epub
2007-00-09
Pages
2955-66
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1855865
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.01596-06 PMC Full Text View journal details
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