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PMID: 12867461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses.

Journal of bacteriology ·Vol. 185 ·No. 15 ·2003-08-00 ·Pages 4519-29

Ishige T, Krause M, Bott M, Wendisch VF, Sahm H

Abstract

The phosphate (P(i)) starvation stimulon of Corynebacterium glutamicum was characterized by global gene expression analysis by using DNA microarrays. Hierarchical cluster analysis of the genes showing altered expression 10 to 180 min after a shift from P(i)-sufficient to P(i)-limiting conditions led to identification of five groups comprising 92 genes. Four of these groups included genes which are not directly involved in P metabolism and changed expression presumably due to the reduced growth rate observed after the shift or to the exchange of medium. One group, however, comprised 25 genes, most of which are obviously related to phosphorus (P) uptake and metabolism and exhibited 4- to >30-fold-greater expression after the shift to P(i) limitation. Among these genes, the RNA levels of the pstSCAB (ABC-type P(i) uptake system), glpQ (glycerophosphoryldiester phosphodiesterase), ugpAEBC (ABC-type sn-glycerol 3-phosphate uptake system), phoH (unknown function), nucH (extracellular nuclease), and Cgl0328 (5'-nucleotidase or related esterase) genes were increased, and pstSCAB exhibited a faster response than the other genes. Transcriptional fusion analyses revealed that elevated expression of pstSCAB and ugpAEBC was primarily due to transcriptional regulation. Several genes also involved in P uptake and metabolism were not affected by P(i) starvation; these included the genes encoding a PitA-like P(i) uptake system and a putative Na(+)-dependent P(i) transporter and the genes involved in the metabolism of pyrophosphate and polyphosphate. In summary, a global, time-resolved picture of the response of C. glutamicum to P(i) starvation was obtained.

MeSH Terms
Bacterial Proteins/genetics,metabolism Cluster Analysis Corynebacterium/genetics,growth & development,physiology Culture Media Gene Expression Profiling Gene Expression Regulation, Bacterial Heat-Shock Response Kinetics Oligonucleotide Array Sequence Analysis Phosphates/metabolism
Chemicals
Bacterial Proteins Culture Media Phosphates
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ishige Takeru
Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany.
Krause Malgorzata
Bott Michael
Wendisch Volker F
Sahm Hermann
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.15.4519-4529.2003
Published
2003-08-00
Pages
4519-29
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC165763
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.15.4519-4529.2003 PMC Full Text View journal details
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