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PMID: 17268547 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Reconstitution and subunit geometry of human condensin complexes.

The EMBO journal ·Vol. 26 ·No. 4 ·2007-02-21 ·Pages 1024-34

Onn I, Aono N, Hirano M, Hirano T

Abstract

Vertebrate cells possess two different condensin complexes, known as condensin I and condensin II, that play a fundamental role in chromosome assembly and segregation during mitosis. Each complex contains a pair of structural maintenance of chromosomes (SMC) ATPases, a kleisin subunit and two HEAT-repeat subunits. Here we use recombinant human condensin subunits to determine their geometry within each complex. We show that both condensin I and condensin II have a pseudo-symmetrical structure, in which the N-terminal half of kleisin links the first HEAT subunit to SMC2, whereas its C-terminal half links the second HEAT subunit to SMC4. No direct interactions are detectable between the SMC dimer and the HEAT subunits, indicating that the kleisin subunit acts as the linchpin in holocomplex assembly. ATP has little, if any, effects on the assembly and integrity of condensin. Cleavage pattern of SMC2 by limited proteolysis is changed upon its binding to ATP or DNA. Our results shed new light on the architecture and dynamics of this highly elaborate machinery designed for chromosome assembly.

MeSH Terms
Adenosine Triphosphatases/chemistry,genetics Amino Acid Sequence Animals Carrier Proteins/metabolism Cell Cycle Proteins Cell Extracts/genetics Chromosome Segregation/genetics DNA-Binding Proteins/chemistry,genetics Genetic Complementation Test Humans Mitosis/genetics Models, Molecular Molecular Sequence Data Multiprotein Complexes/chemistry,genetics Nuclear Proteins/metabolism Ovum/chemistry Protein Conformation Protein Subunits/chemistry,metabolism Xenopus
Chemicals
Carrier Proteins Cell Cycle Proteins Cell Extracts DNA-Binding Proteins Multiprotein Complexes Nuclear Proteins Protein Subunits SMC2 protein, human condensin complexes Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Onn Itay
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Aono Nobuki
Hirano Michiko
Hirano Tatsuya
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39 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7601562
Published
2007-02-21
Epub
2007-00-01
Pages
1024-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1852836
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7601562 PMC Full Text View journal details
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