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PMID: 12773391 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly.

The EMBO journal ·Vol. 22 ·No. 11 ·2003-06-02 ·Pages 2764-75

Sakai A, Hizume K, Sutani T, Takeyasu K, Yanagida M

Abstract

Condensin and cohesin are chromosomal protein complexes required for chromosome condensation and sister chromatid cohesion, respectively. They commonly contain the SMC (structural maintenance of chromosomes) subunits consisting of a long coiled-coil with the terminal globular domains and the central hinge. Condensin and cohesin holo-complexes contain three and two non-SMC subunits, respectively. In this study, DNA interaction with cohesin and condensin complexes purified from fission yeast was investigated. The DNA reannealing activity is strong for condensin SMC heterodimer but weak for holo-condensin, whereas no annealing activity is found for cohesin heterodimer SMC and Rad21-bound heterotrimer complexes. One set of globular domains of the same condensin SMC is essential for the DNA reannealing activity. In addition, the coiled-coil and hinge region of another SMC are needed. Atomic force microscopy discloses the molecular events of DNA reannealing. SMC assembly that occurs on reannealing DNA seems to be a necessary intermediary step. SMC is eliminated from the completed double-stranded DNA. The ability of heterodimeric SMC to reanneal DNA may be regulated in vivo possibly through the non-SMC heterotrimeric complex.

MeSH Terms
Adenosine Triphosphatases/chemistry,metabolism Cell Cycle Proteins Chromatin/metabolism Chromosomal Proteins, Non-Histone DNA, Fungal/chemistry,genetics,metabolism DNA-Binding Proteins/chemistry,metabolism Dimerization Fungal Proteins Multiprotein Complexes Nuclear Proteins/chemistry,metabolism Nucleic Acid Conformation Phosphoproteins/metabolism Protein Binding Protein Structure, Quaternary Protein Structure, Tertiary Protein Subunits Recombinant Proteins/chemistry,metabolism Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins/chemistry,metabolism
Chemicals
Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA, Fungal DNA-Binding Proteins Fungal Proteins Multiprotein Complexes Nuclear Proteins Phosphoproteins Protein Subunits Rad21 protein, S pombe Recombinant Proteins Schizosaccharomyces pombe Proteins cohesins condensin complexes Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sakai Akiko
Department of Biophysics, Graduate School of Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
Hizume Kohji
Sutani Takashi
Takeyasu Kunio
Yanagida Mitsuhiro
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg247
Published
2003-06-02
Pages
2764-75
Language
English
Region
England
NLM ID
8208664
PMCID
PMC156744
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg247 PMC Full Text View journal details
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