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PMID: 1717488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells.

The Journal of cell biology ·Vol. 115 ·No. 2 ·1991-10-00 ·Pages 557-64

Zhou Q, Moore KL, Smith DF, Varki A, McEver RP, Cummings RD

Abstract

Granule membrane protein-140 (GMP-140) is an inducible receptor for myeloid leukocytes on activated platelets and endothelium. Like other selectins, GMP-140 recognizes specific oligosaccharide ligands. However, prior data on the nature of these ligands are contradictory. We investigated the structural features required for ligand interaction with GMP-140 using purified GMP-140, cells naturally expressing specific oligosaccharides, and cells expressing cloned glycosyltransferases. Like the related selectin endothelial leukocyte adhesion molecule-1 (ELAM-1), GMP-140 recognizes alpha(2-3)sialylated, alpha(1-3)fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells, including the sequence Neu5Ac alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNac beta-R (sialyl Lewis x). Recognition requires sialic acid, because cells expressing large amounts of Lewis x, but not sialyl Lewis x, do not interact with GMP-140. Although sialyl Lewis x is expressed by both myeloid HL-60 cells and CHO cells transfected with an alpha 1-3/4 fucosyltransferase, GMP-140 binds with significantly higher affinity to HL-60 cells. Thus, the sialyl Lewis x tetrasaccharide may require additional structural modifications or specific presentations in order for leukocytes in flowing blood to interact rapidly and with high affinity to GMP-140 on activated platelets or endothelium.

MeSH Terms
Amino Sugars/metabolism Animals Antibodies, Monoclonal/metabolism CHO Cells Carbohydrate Sequence Cell Line Cricetinae Fucose/metabolism Humans Lewis X Antigen/metabolism Molecular Sequence Data Neuraminidase/genetics,metabolism P-Selectin Platelet Membrane Glycoproteins/metabolism Polysaccharides/metabolism Sialic Acids/metabolism Transfection/genetics Tumor Cells, Cultured
Chemicals
Amino Sugars Antibodies, Monoclonal Lewis X Antigen P-Selectin Platelet Membrane Glycoproteins Polysaccharides Sialic Acids lactosaminoglycan Fucose Neuraminidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhou Q
Department of Biochemistry, University of Georgia, Athens 30602.
Moore K L
Smith D F
Varki A
McEver R P
Cummings R D
References (62)
62 references, click to expand
  1. Analysis and purification of oligosaccharides by high-performance liquid affinity chromatography.
    Methods Enzymol. 1989;179:55-64 PMID: 2622361
  2. The spectrum of anionic oligosaccharides released by endo-beta-N-acetylglucosaminidase H from glycoproteins. Structural studies and interactions with the phosphomannosyl receptor.
    J Biol Chem. 1983 Mar 10;258(5):2808-18 PMID: 6298207
  3. Purification and properties of a Ca2+-independent sialic acid-binding lectin from human placenta with preferential affinity to O-acetylsialic acids.
    J Biol Chem. 1989 Nov 5;264(31):18673-8 PMID: 2808392
  4. Asparagine-linked oligosaccharides containing poly-N-acetyllactosamine chains are preferentially bound by immobilized calf heart agglutinin.
    J Biol Chem. 1988 Nov 5;263(31):16143-9 PMID: 3182789
  5. Characterization of sialosylated Lewisx as a new tumor-associated antigen.
    Cancer Res. 1984 Nov;44(11):5279-85 PMID: 6386148
  6. Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes.
    J Biol Chem. 1986 Jan 5;261(1):96-100 PMID: 3510203
  7. Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by a human erythropoietin cDNA.
    J Biol Chem. 1987 Sep 5;262(25):12059-76 PMID: 3624248
  8. The action of sialidases on substrates containing O-acetylsialic acids.
    Biol Chem Hoppe Seyler. 1986 May;367(5):433-9 PMID: 3741623
  9. Purification and characterization of an O-acetylsialic acid-specific lectin from a marine crab Cancer antennarius.
    J Biol Chem. 1985 Jul 25;260(15):8850-6 PMID: 4019458
  10. Involvement of sialic acid on endothelial cells in organ-specific lymphocyte recirculation.
    Science. 1985 May 24;228(4702):1005-7 PMID: 4001928
  11. Membrane mutants of animal cells: rapid identification of those with a primary defect in glycosylation.
    Mol Cell Biol. 1985 May;5(5):923-9 PMID: 4000122
  12. Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor.
    J Cell Biol. 1990 Sep;111(3):1225-32 PMID: 2202735
  13. The S-type lectin from calf heart tissue binds selectively to the carbohydrate chains of laminin.
    Arch Biochem Biophys. 1990 Aug 15;281(1):27-35 PMID: 1696449
  14. Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions.
    Proc Natl Acad Sci U S A. 1990 Mar;87(5):1673-7 PMID: 1689848
  15. PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15).
    Cell. 1990 Nov 2;63(3):467-74 PMID: 1699666
  16. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins.
    Cell. 1991 May 31;65(5):859-73 PMID: 1710173
  17. Carbohydrate ligands for endothelial-leukocyte adhesion molecule 1.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1138-42 PMID: 1705026
  18. Selectins: novel receptors that mediate leukocyte adhesion during inflammation.
    Thromb Haemost. 1991 Mar 4;65(3):223-8 PMID: 1710835
  19. Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells.
    Science. 1990 Nov 23;250(4984):1132-5 PMID: 1701275
  20. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex.
    Science. 1990 Nov 23;250(4984):1130-2 PMID: 1701274
  21. ELAM-1--dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA.
    Cell. 1990 Nov 2;63(3):475-84 PMID: 1699667
  22. Carbohydrate ligands of the LEC cell adhesion molecules.
    Cell. 1990 Nov 30;63(5):861-3 PMID: 1701693
  23. GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction.
    J Cell Biol. 1991 Feb;112(3):491-9 PMID: 1704009
  24. ELFT: a gene that directs the expression of an ELAM-1 ligand.
    Cell. 1990 Dec 21;63(6):1349-56 PMID: 1702034
  25. Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets.
    Biochem Biophys Res Commun. 1990 Nov 15;172(3):1349-56 PMID: 1700907
  26. Structure of sialylated fucosyl lactosaminoglycan isolated from human granulocytes.
    J Biol Chem. 1984 Sep 10;259(17):10925-35 PMID: 6432790
  27. A monoclonal antibody to a membrane glycoprotein binds only to activated platelets.
    J Biol Chem. 1984 Aug 10;259(15):9799-804 PMID: 6746667
  28. A platelet membrane protein expressed during platelet activation and secretion. Studies using a monoclonal antibody specific for thrombin-activated platelets.
    J Biol Chem. 1984 Jul 25;259(14):9121-6 PMID: 6746643
  29. Methods for binding cells to plastic: application to solid phase immunoassays for cell-surface antigens.
    Methods Enzymol. 1981;73(Pt B):406-18 PMID: 7300685
  30. An autosomal dominant gene regulates the extent of 9-O-acetylation of murine erythrocyte sialic acids. A probable explanation for the variation in capacity to activate the human alternate complement pathway.
    J Exp Med. 1980 Sep 1;152(3):532-44 PMID: 7411019
  31. Structure of an unusual complex-type oligosaccharide isolated from Chinese hamster ovary cells.
    Arch Biochem Biophys. 1980 Feb;199(2):393-9 PMID: 7362235
  32. Vertebrate lectins, Comparison of properties of beta-galactoside-binding lectins from tissues of calf and chicken.
    J Cell Biol. 1979 Jun;81(3):528-37 PMID: 379020
  33. Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism: characterization of Chinese hamster cell mutants defective in phosphoribosylpyrophosphate amidotransferase and phosphoribosylglycinamide synthetase and an examination of alternatives to the first step of purine biosynthesis.
    Somatic Cell Genet. 1977 Nov;3(6):561-77 PMID: 564086
  34. The dysfunctional LDL receptor in a monensin-resistant mutant of Chinese hamster ovary cells lacks selected O-linked oligosaccharides.
    Arch Biochem Biophys. 1991 Feb 1;284(2):245-56 PMID: 1899178
  35. Cell adhesion. Sticky sugars for selectins.
    Nature. 1991 Jan 17;349(6306):196-7 PMID: 1987472
  36. Requirement for sialic acid on the endothelial ligand of a lymphocyte homing receptor.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2757-64 PMID: 2277086
  37. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.
    Cell. 1989 Oct 20;59(2):305-12 PMID: 2478294
  38. Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains.
    Science. 1989 Mar 3;243(4895):1165-72 PMID: 2646713
  39. Alteration of terminal glycosylation sequences on N-linked oligosaccharides of Chinese hamster ovary cells by expression of beta-galactoside alpha 2,6-sialyltransferase.
    J Biol Chem. 1989 Aug 15;264(23):13848-55 PMID: 2668274
  40. Retinoic acid-induced differentiation of the mouse teratocarcinoma cell line F9 is accompanied by an increase in the activity of UDP-galactose: beta-D-galactosyl-alpha 1,3-galactosyltransferase.
    J Biol Chem. 1988 Jan 5;263(1):511-9 PMID: 3121614
  41. Influenza virus hemagglutinins differentiate between receptor determinants bearing N-acetyl-, N-glycollyl-, and N,O-diacetylneuraminic acids.
    Virology. 1985 Jul 15;144(1):279-82 PMID: 4060591
  42. Structures of sialylated fucosyl polylactosaminoglycans isolated from chronic myelogenous leukemia cells.
    J Biol Chem. 1985 Oct 25;260(24):12957-67 PMID: 3863814
  43. Identification of an inducible endothelial-leukocyte adhesion molecule.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9238-42 PMID: 2827173
  44. Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids.
    Am J Hum Genet. 1986 Jun;38(6):793-804 PMID: 3014865
  45. A platelet alpha granule membrane protein that is associated with the plasma membrane after activation. Characterization and subcellular localization of platelet activation-dependent granule-external membrane protein.
    J Clin Invest. 1986 Jul;78(1):130-7 PMID: 2941452
  46. Two distinct classes of carbohydrate-recognition domains in animal lectins.
    J Biol Chem. 1988 Jul 15;263(20):9557-60 PMID: 3290208
  47. GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies.
    J Clin Invest. 1989 Jul;84(1):92-9 PMID: 2472431
  48. PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells.
    Blood. 1989 Apr;73(5):1109-12 PMID: 2467701
  49. Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP-140.
    J Biol Chem. 1989 May 15;264(14):7768-71 PMID: 2470733
  50. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation.
    Cell. 1989 Mar 24;56(6):1033-44 PMID: 2466574
  51. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.
    Science. 1989 Mar 3;243(4895):1160-5 PMID: 2466335
  52. Multiple soluble beta-galactoside-binding lectins from human lung.
    J Biol Chem. 1987 May 25;262(15):7383-90 PMID: 2438278
  53. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation.
    J Cell Biol. 1985 Sep;101(3):880-6 PMID: 2411738
  54. Cloning of a lymphocyte homing receptor reveals a lectin domain.
    Cell. 1989 Mar 24;56(6):1045-55 PMID: 2647302
  55. Rapid neutrophil adhesion to activated endothelium mediated by GMP-140.
    Nature. 1990 Feb 22;343(6260):757-60 PMID: 1689464
  56. GMP-140 mediates adhesion of stimulated platelets to neutrophils.
    Blood. 1990 Feb 1;75(3):550-4 PMID: 1688717
  57. Studies of naturally occurring modifications of sialic acids by fast-atom bombardment-mass spectrometry. Analysis of positional isomers by periodate cleavage.
    J Biol Chem. 1990 May 15;265(14):8094-107 PMID: 2159468
  58. Transfer and expression of a murine UDP-Gal:beta-D-Gal-alpha 1,3-galactosyltransferase gene in transfected Chinese hamster ovary cells. Competition reactions between the alpha 1,3-galactosyltransferase and the endogenous alpha 2,3-sialyltransferase.
    J Biol Chem. 1990 Apr 15;265(11):6225-34 PMID: 2108155
  59. Radioiodination of proteins by the use of the chloramine-T method.
    Methods Enzymol. 1980;70(A):210-3 PMID: 7421589
  60. O-acetylation of disialoganglioside GD3 by human melanoma cells creates a unique antigenic determinant.
    Science. 1984 Aug 24;225(4664):844-6 PMID: 6206564
  61. Monoclonal antibody specific for granulocytic-lineage cells and reactive with human pluripotent and committed haematopoietic progenitor cells.
    Br J Haematol. 1984 Sep;58(1):159-68 PMID: 6205678
  62. An inducible endothelial cell surface glycoprotein mediates melanoma adhesion.
    Science. 1989 Dec 8;246(4935):1303-6 PMID: 2588007
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.115.2.557
Published
1991-10-00
Pages
557-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289144
Subset
IM
Grants
NCI NIH HHS · CA 38701 · United States
NHLBI NIH HHS · HL 34363 · United States
NHLBI NIH HHS · HL 45510 · United States
External Links
PubMed source DOI: 10.1083/jcb.115.2.557 PMC Full Text View journal details
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