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PMID: 1716615 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct expression of Bordetella pertussis filamentous hemagglutinin in Escherichia coli and Salmonella typhimurium aroA.

Infection and immunity ·Vol. 59 ·No. 10 ·1991-10-00 ·Pages 3787-95

Guzmán CA, Walker MJ, Rohde M, Timmis KN

Abstract

Nonfused (i.e., nonhybrid) filamentous hemagglutinin (FHA) of Bordetella pertussis was efficiently expressed in Escherichia coli K-12 and Salmonella typhimurium aroA at levels higher than those found in wild-type B. pertussis when the upstream signals of the gene were replaced and the translation initiation region was engineered to optimize translational efficiency. Inclusion of part of the C-terminal FHA open reading frame, whose translation product does not appear to be part of the major secreted species of FHA, was shown to be important in achieving protein expression in both E. coli and S. typhimurium aroA; removal of the downstream gene sequence abolished recombinant FHA production. The levels of expression observed varied widely according to the construct and host bacterium used.

MeSH Terms
Adhesins, Bacterial Antibodies, Monoclonal Base Sequence Bordetella pertussis/genetics Chromosome Mapping Epitopes Escherichia coli/genetics Hemagglutinins/biosynthesis,genetics Molecular Sequence Data Plasmids Protein Biosynthesis Salmonella typhimurium/genetics Virulence Factors, Bordetella
Chemicals
Adhesins, Bacterial Antibodies, Monoclonal Epitopes Hemagglutinins Virulence Factors, Bordetella filamentous hemagglutinin adhesin, Bordetella pertussis
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guzmán C A
Department of Microbiology, GBF-National Research Center for Biotechnology, Braunschweig, Germany.
Walker M J
Rohde M
Timmis K N
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.10.3787-3795.1991
Published
1991-10-00
Pages
3787-95
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258952
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.10.3787-3795.1991 PMC Full Text View journal details
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