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PMID: 17158956 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin.

The Journal of cell biology ·Vol. 175 ·No. 6 ·2006-12-18 ·Pages 1005-15

Wong CE, Paratore C, Dours-Zimmermann MT, Rochat A, Pietri T, Suter U, Zimmermann DR, Dufour S, Thiery JP, Meijer D, Beermann F, Barrandon Y, Sommer L

Abstract

Given their accessibility, multipotent skin-derived cells might be useful for future cell replacement therapies. We describe the isolation of multipotent stem cell-like cells from the adult trunk skin of mice and humans that express the neural crest stem cell markers p75 and Sox10 and display extensive self-renewal capacity in sphere cultures. To determine the origin of these cells, we genetically mapped the fate of neural crest cells in face and trunk skin of mouse. In whisker follicles of the face, many mesenchymal structures are neural crest derived and appear to contain cells with sphere-forming potential. In the trunk skin, however, sphere-forming neural crest-derived cells are restricted to the glial and melanocyte lineages. Thus, self-renewing cells in the adult skin can be obtained from several neural crest derivatives, and these are of distinct nature in face and trunk skin. These findings are relevant for the design of therapeutic strategies because the potential of stem and progenitor cells in vivo likely depends on their nature and origin.

MeSH Terms
Adipocytes/cytology,metabolism Adult Animals Cell Differentiation Cell Lineage Cells, Cultured DNA-Binding Proteins/metabolism Face Female Fluorescent Antibody Technique Hair Follicle/cytology,physiology High Mobility Group Proteins/metabolism Humans Male Melanocytes/cytology,physiology Mice Mice, Inbred C57BL Middle Aged Multipotent Stem Cells/cytology,physiology Neural Crest/cytology,physiology Neuroglia/cytology,physiology SOXE Transcription Factors Skin/cytology Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins High Mobility Group Proteins SOX10 protein, human SOXE Transcription Factors Sox10 protein, mouse Transcription Factors
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Wong Christine E
Department of Biology, Institute of Cell Biology, Swiss Federal Institute of Technology ETH Zurich, CH-8093 Zurich, Switzerland.
Paratore Christian
Dours-Zimmermann María T
Rochat Ariane
Pietri Thomas
Suter Ueli
Zimmermann Dieter R
Dufour Sylvie
Thiery Jean Paul
Meijer Dies
Beermann Friedrich
Barrandon Yann
Sommer Lukas
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200606062
Published
2006-12-18
Epub
2006-00-11
Pages
1005-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064709
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200606062 PMC Full Text View journal details
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