The cps gene cluster of Salmonella strain LT2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster.

Molecular & general genetics : MGG ·Vol. 227 ·No. 2 ·1991-06-00 ·Pages 173-80

Stevenson G, Lee SJ, Romana LK, Reeves PR

Abstract

We report the presence in Salmonella enterica strain LT2 (serovar thyphimurium) of duplicate genes for two steps in the synthesis of GDP-mannose. The previously known genes, rfbK (phosphomannomutase) and rfbM (mannose-1-phosphate guanyltransferase), are part of the gene cluster for the O antigen. The two new genes, cpsB and cpsG, respectively, are thought to be part of the gene cluster for the M antigen capsular polysaccharide, present in many Enterobacteriaceae. The two genes have been sequenced and have a GC content of 0.61, suggesting an origin outside of Salmonella. Comparison of the inferred protein sequences for cpsB and rfbM shows 57% identity of amino acids whereas for cpsG and rfbK there is only 19% identity. It is suggested that the greater divergence between cpsG and rfbK may be due to a period of accelerated evolution, perhaps precipitated by transfer of the genes from another species.

Related Genes

cpsB cpsG rfbK rfbM

MeSH Terms

Amino Acid Sequence Base Composition Base Sequence Cloning, Molecular Gene Frequency Guanosine Diphosphate Mannose/metabolism Isoenzymes/genetics Isomerases/genetics Molecular Sequence Data Multigene Family Nucleotidyltransferases/genetics Phosphotransferases (Phosphomutases) Polysaccharides, Bacterial Salmonella/enzymology,genetics,immunology Sequence Homology, Nucleic Acid

Chemicals

Isoenzymes Polysaccharides, Bacterial Guanosine Diphosphate Mannose Nucleotidyltransferases mannose-1-phosphate guanylyltransferase (GDP) Isomerases Phosphotransferases (Phosphomutases) phosphomannomutase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Stevenson G

Department of Microbiology, University of Sydney, N.S.W., Australia.

Lee S J

Romana L K

Reeves P R

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20 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00259668

Published

1991-06-00

Pages

173-80

Language

English

Region

Germany

NLM ID

0125036

Subset

Databases

GENBANK

X54103, X59886, X63980, X63981, X63982, X63983, X63984, X63985, X63986, X63987

External Links

PubMed source DOI: 10.1007/BF00259668

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The cps gene cluster of Salmonella strain LT2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster.

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