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PMID: 1712012 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of a retroelement with a P4-like cryptic prophage (retronphage phi R73) integrated into the selenocystyl tRNA gene of Escherichia coli.

Journal of bacteriology ·Vol. 173 ·No. 13 ·1991-07-00 ·Pages 4171-81

Sun J, Inouye M, Inouye S

Abstract

A new multicopy single-stranded DNA (msDNA-Ec73) was found in a clinical strain of Escherichia coli. Retron-Ec73, consisting of an msDNA-coding region and the gene for reverse transcriptase (RT), was found to be a part of a 12.7-kb foreign DNA fragment flanked by 29-bp direct repeats and integrated into the gene for selenocystyl-tRNA (selC) at 82 min on the E. coli chromosome. Except for the 2.4-kb retron region, the integrated DNA fragment showed remarkable homology to most of the bacteriophage P4 genome. Among the phage genes found in this element, however, the integrase gene had very low identity (40%) to P4 integrase, indicating that the cryptic prophage associated with the retroelement has its own unique site-specific integrase different from P4 integrase. Recently, we have shown that P2 phage can act as a helper to excise the cryptic prophage and to package its genome into an infectious virion. The newly formed phage (retronphage phi R73) can also lysogenize a new host strain, reintegrating its genome into the selC gene and enabling the newly formed lysogen to produce msDNA-Ec73 (S. Inouye, M. G. Sunshine, E. W. Six, and M. Inouye, Science 252:969-971, 1991).

Related Genes
selC
MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Chromosomes, Bacterial Coliphages/genetics DNA Nucleotidyltransferases/metabolism DNA Transposable Elements DNA, Single-Stranded/chemistry Escherichia coli/enzymology,genetics Genes, Bacterial Integrases Lysogeny Molecular Sequence Data Nucleic Acid Conformation Protein Biosynthesis RNA, Transfer, Amino Acid-Specific/genetics RNA-Directed DNA Polymerase/genetics Sequence Homology, Nucleic Acid Substrate Specificity
Chemicals
DNA Transposable Elements DNA, Single-Stranded RNA, Transfer, Amino Acid-Specific tRNA, selenocysteine- DNA Nucleotidyltransferases Integrases RNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sun J
Department of Biochemistry, University of Medicine and Dentistry of New Jersey at Rutgers, Piscataway 08854-5635.
Inouye M
Inouye S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.13.4171-4181.1991
Published
1991-07-00
Pages
4171-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208067
Subset
IM
Grants
NIGMS NIH HHS · GM44012 · United States
Databases
GENBANK
M64113, M74477, M74478, M74479, M74480, M74481, M74482, M74483, M74484, S38733
External Links
PubMed source DOI: 10.1128/jb.173.13.4171-4181.1991 PMC Full Text View journal details
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