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PMID: 17116695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans.

Molecular and cellular biology ·Vol. 27 ·No. 3 ·2007-02-00 ·Pages 791-802

Bernreiter A, Ramon A, Fernández-Martínez J, Berger H, Araújo-Bazan L, Espeso EA, Pachlinger R, Gallmetzer A, Anderl I, Scazzocchio C, Strauss J

Abstract

NirA, the specific transcription factor of the nitrate assimilation pathway of Aspergillus nidulans, accumulates in the nucleus upon induction by nitrate. NirA interacts with the nuclear export factor KapK, which bridges an interaction with a protein of the nucleoporin-like family (NplA). Nitrate induction disrupts the NirA-KapK interaction in vivo, whereas KapK associates with NirA when this protein is exported from the nucleus. A KpaK leptomycin-sensitive mutation leads to inducer-independent NirA nuclear accumulation in the presence of the drug. However, this does not lead to constitutive expression of the genes controlled by NirA. A nirA(c)1 mutation leads to constitutive nuclear localization and activity, remodeling of chromatin, and in vivo binding to a NirA upstream activation sequence. The nirA(c)1 mutation maps in the nuclear export signal (NES) of the NirA protein. The NirA-KapK interaction is nearly abolished in NirA(c)1 and NirA proteins mutated in canonical leucine residues in the NirA NES. The latter do not result in constitutively active NirA protein, which implies that nuclear retention is necessary but not sufficient for NirA activity. The results are consistent with a model in which activation of NirA by nitrate disrupts the interaction of NirA with the NplA/KapK nuclear export complex, thus resulting in nuclear retention, leading to AreA-facilitated DNA binding of the NirA protein and subsequent chromatin remodeling and transcriptional activation.

MeSH Terms
Active Transport, Cell Nucleus/drug effects Aspergillus nidulans/cytology,drug effects,metabolism Binding Sites/drug effects Cell Nucleus/drug effects,metabolism Fatty Acids, Unsaturated/pharmacology Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal/drug effects Karyopherins/metabolism Mutation/genetics Nitrates/metabolism Nuclear Export Signals/drug effects Nuclear Pore Complex Proteins/metabolism Phenotype Protein Binding/drug effects RNA, Messenger/genetics,metabolism Receptors, Cytoplasmic and Nuclear/metabolism Recombinant Fusion Proteins/metabolism Sequence Homology Transcription Factors/genetics,metabolism Two-Hybrid System Techniques
Chemicals
Fatty Acids, Unsaturated Fungal Proteins Karyopherins Nitrates Nuclear Export Signals Nuclear Pore Complex Proteins RNA, Messenger Receptors, Cytoplasmic and Nuclear Recombinant Fusion Proteins Transcription Factors exportin 1 protein NirA protein, Aspergillus nidulans leptomycin B
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Bernreiter Andreas
Fungal Genetics and Genomics Unit, Austrian Research Centers and BOKU Vienna, Muthgasse 18, A-1190 Vienna, Austria.
Ramon Ana
Fernández-Martínez Javier
Berger Harald
Araújo-Bazan Lidia
Espeso Eduardo A
Pachlinger Robert
Gallmetzer Andreas
Anderl Ingund
Scazzocchio Claudio
Strauss Joseph
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
2007-02-00
Epub
2006-00-20
Pages
791-802
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1800680
Subset
IM
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