Home LiteratureArticle Details
PMID: 9685506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.

Nucleic acids research ·Vol. 26 ·No. 16 ·1998-08-15 ·Pages 3862-4

Wolschek MF, Narendja F, Karlseder J, Kubicek CP, Scazzocchio C, Strauss J

Abstract

The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.

MeSH Terms
Aspergillus nidulans/genetics,metabolism Aspergillus niger/genetics,metabolism Base Sequence Binding Sites/genetics DNA Footprinting/methods DNA Methylation DNA, Fungal/genetics,isolation & purification,metabolism Fungal Proteins/metabolism Genes, Fungal Mitosporic Fungi/genetics,metabolism Oligonucleotide Probes/genetics Penicillium chrysogenum/genetics,metabolism Polymerase Chain Reaction Promoter Regions, Genetic Protein Binding
Chemicals
DNA, Fungal Fungal Proteins Oligonucleotide Probes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wolschek M F
IBTM, Technical University Vienna, Getreidemarkt 9/172-5, A-1060 Vienna, Austria, Institut für Biochemie,Vienna Biocenter, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.
Narendja F
Karlseder J
Kubicek C P
Scazzocchio C
Strauss J
References (13)
13 references, click to expand
  1. The genetics of Aspergillus nidulans.
    Adv Genet. 1953;5:141-238 PMID: 13040135
  2. Cloning of the regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans.
    EMBO J. 1986 May;5(5):1087-90 PMID: 3013617
  3. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR.
    Science. 1989 Nov 10;246(4931):780-6 PMID: 2814500
  4. The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger.
    EMBO J. 1990 May;9(5):1355-64 PMID: 1970293
  5. nirA, the pathway-specific regulatory gene of nitrate assimilation in Aspergillus nidulans, encodes a putative GAL4-type zinc finger protein and contains four introns in highly conserved regions.
    Mol Cell Biol. 1991 Nov;11(11):5746-55 PMID: 1922075
  6. Localization of growth and secretion of proteins in Aspergillus niger.
    J Gen Microbiol. 1991 Aug;137(8):2017-23 PMID: 1955876
  7. The regulator of nitrate assimilation in ascomycetes is a dimer which binds a nonrepeated, asymmetrical sequence.
    Mol Cell Biol. 1998 Mar;18(3):1339-48 PMID: 9488449
  8. Molecular characterization of a fungal secondary metabolism promoter: transcription of the Aspergillus nidulans isopenicillin N synthetase gene is modulated by upstream negative elements.
    Mol Microbiol. 1993 Aug;9(4):881-95 PMID: 8231816
  9. The intergenic region between the divergently transcribed niiA and niaD genes of Aspergillus nidulans contains multiple NirA binding sites which act bidirectionally.
    Mol Cell Biol. 1995 Oct;15(10):5688-99 PMID: 7565720
  10. NRE, the major nitrogen regulatory protein of Penicillium chrysogenum, binds specifically to elements in the intergenic promoter regions of nitrate assimilation and penicillin biosynthetic gene clusters.
    Curr Genet. 1995 Jul;28(2):177-83 PMID: 8590470
  11. Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA.
    EMBO J. 1997 Jul 1;16(13):3974-86 PMID: 9233807
  12. The molecular biology of secreted enzyme production by fungi.
    Crit Rev Biotechnol. 1997;17(4):273-306 PMID: 9397531
  13. The Aspergillus niger niaD gene encoding nitrate reductase: upstream nucleotide and amino acid sequence comparisons.
    Gene. 1992 Feb 15;111(2):149-55 PMID: 1541396
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.16.3862
Published
1998-08-15
Pages
3862-4
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147760
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/26.16.3862 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2