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PMID: 17114265 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Bacillus subtilis genome diversity.

Journal of bacteriology ·Vol. 189 ·No. 3 ·2007-02-00 ·Pages 1163-70

Earl AM, Losick R, Kolter R

Abstract

Microarray-based comparative genomic hybridization (M-CGH) is a powerful method for rapidly identifying regions of genome diversity among closely related organisms. We used M-CGH to examine the genome diversity of 17 strains belonging to the nonpathogenic species Bacillus subtilis. Our M-CGH results indicate that there is considerable genetic heterogeneity among members of this species; nearly one-third of Bsu168-specific genes exhibited variability, as measured by the microarray hybridization intensities. The variable loci include those encoding proteins involved in antibiotic production, cell wall synthesis, sporulation, and germination. The diversity in these genes may reflect this organism's ability to survive in diverse natural settings.

MeSH Terms
Bacillus subtilis/genetics Bacterial Proteins/genetics Genes, Bacterial Genetic Variation Genome, Bacterial Nucleic Acid Hybridization/methods Oligonucleotide Array Sequence Analysis Phylogeny Species Specificity
Chemicals
Bacterial Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Earl Ashlee M
Department of Microbiology & Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Losick Richard
Kolter Roberto
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.01343-06
Published
2007-02-00
Epub
2006-00-17
Pages
1163-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1797320
Subset
IM
Grants
NIGMS NIH HHS · F32 GM072393 · United States
NIGMS NIH HHS · R01 GM058213 · United States
NIGMS NIH HHS · GM072393 · United States
NIGMS NIH HHS · GM58213 · United States
External Links
PubMed source DOI: 10.1128/JB.01343-06 PMC Full Text View journal details
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