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PMID: 1710618 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A novel transcriptional response by the cat gene during slow growth of Escherichia coli.

Journal of bacteriology ·Vol. 173 ·No. 11 ·1991-06-00 ·Pages 3523-30

Meyer BJ, Schottel JL

Abstract

A novel response to growth rate was found with expression of the chloramphenicol acetyltransferase (cat) gene in Escherichia coli. The amount of cat mRNA relative to total RNA increased about 11-fold as growth rates decreased 5- to 6-fold, without an increase in translation. The accumulation of cat mRNA was in contrast to decreased cellular concentrations of total RNA, trxA, ompA, or 23S rRNA as the growth rate decreased and was not due to changes in gene dosage or mRNA stability. Stability of the cat mRNA does not appear to be regulated by growth rate. No significant change in either chemical or functional stability was observed within a five- to sixfold range of growth rates when chemostat-grown cells were used. However, cat mRNA stability was affected by growth medium composition. The half-life of cat mRNA decreased about threefold, with an approximate fourfold increase in generation time due to changes in growth medium. Transcriptional studies have indicated that accumulation of cat mRNA at slow growth rates is the result of a specific transcriptional response to changes in cellular generation times. We propose that increases in the cellular concentration of a specific message at slow growth rates may reflect an additional type of survival response in E. coli.

Related Genes
cat ompA trxA
MeSH Terms
Cell Division/genetics Chloramphenicol O-Acetyltransferase/biosynthesis,genetics DNA Probes Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Gene Expression Regulation, Bacterial Half-Life Immunoblotting RNA/analysis RNA, Messenger/analysis Transcription, Genetic/physiology
Chemicals
DNA Probes RNA, Messenger RNA Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Meyer B J
Department of Microbiology, University of Minnesota, Minneapolis 55455.
Schottel J L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.11.3523-3530.1991
Published
1991-06-00
Pages
3523-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207967
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.11.3523-3530.1991 PMC Full Text View journal details
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