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PMID: 1710339 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Calpastatin and nucleotides stabilize cardiac calcium channel activity in excised patches.

Pflugers Archiv : European journal of physiology ·Vol. 418 ·No. 1-2 ·1991-03-00 ·Pages 86-92

Romanin C, Grösswagen P, Schindler H

Abstract

The activity of single L-type Ca2+ channels is rapidly lost (run-down) when contact between the membrane and cytosol is interrupted. We have now achieved the stabilization of cardiac Ca2+ channel activity of guinea-pig ventricular myocytes by using either cytosol or defined components added to excised patches. The endogenous protease inhibitor, calpastatin, together with nucleotides, ATP + GTP, was found to prevent run-down as effectively as cardiac cytosolic solution. These results suggest the involvement of proteolysis by calpain in run-down of channel activity and enable the study of cardiac Ca2+ channel regulation with free access to both sides of the membrane.

MeSH Terms
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology Adenosine Triphosphate/pharmacology Animals Calcium Channels/drug effects,metabolism Calcium-Binding Proteins/pharmacology Cysteine Proteinase Inhibitors/pharmacology Electric Conductivity Guanosine Triphosphate/pharmacology Guinea Pigs In Vitro Techniques Myocardium/metabolism
Chemicals
Calcium Channels Calcium-Binding Proteins Cysteine Proteinase Inhibitors 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester calpastatin Guanosine Triphosphate Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Romanin C
Institute for Biophysics, University of Linz, Austria.
Grösswagen P
Schindler H
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36 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00370456
Published
1991-03-00
Pages
86-92
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00370456 View journal details
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