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PMID: 1709495 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The murine 2-5A synthetase locus: three distinct transcripts from two linked genes.

Nucleic acids research ·Vol. 19 ·No. 8 ·1991-04-25 ·Pages 1917-24

Rutherford MN, Kumar A, Nissim A, Chebath J, Williams BR

Abstract

The cloning of cDNAs encoding murine 2-5A synthetase has identified three related transcripts, represented by a previously described cDNA clone, L3 and two novel cDNAs, L1 and L2. L1 contains an open reading frame coding for a protein that shows 70% conservation at the amino acid level when compared to the protein predicted to be encoded by L3. L2 recognizes an IFN-induced transcript 600-bp larger than the L3 transcript. These three cDNAs map to a cosmid, cII, containing two murine 2-5A synthetase genes, ME12 and ME5/ME8, situated in a head-to-tail orientation separated by approximately 8 kb. Southern analyses of ME12 and ME5/ME8 using L3, L1-specific and L2-specific probes indicate that these genes have a similar organization. cII was transiently and stably transfected into CV-1 cells. When treated with interferon, the transfected cells produced mature, murine 2-5A synthetase transcripts identified using L3 and L2-specific probes. Thus all transcripts present in IFN-treated mouse cells which are recognized by the available murine 2-5A synthetase cDNA probes map to an approximately 40 kb region of the mouse genome containing two 2-5A synthetase genes.

Related Genes
ME12 ME5/ME8
MeSH Terms
2',5'-Oligoadenylate Synthetase/genetics,metabolism Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern Cell Line DNA Genetic Linkage Genomic Library Interferons/pharmacology Mice Molecular Sequence Data Open Reading Frames Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic Transfection
Chemicals
DNA Interferons 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rutherford M N
Research Institute, Hospital for Sick Children, Toronto, Canada.
Kumar A
Nissim A
Chebath J
Williams B R
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.8.1917
Published
1991-04-25
Pages
1917-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328124
Subset
IM
Databases
GENBANK
X15928, X15929, X15930, X15931, X15932, X15933, X15934, X15935, X55982, X58077
External Links
PubMed source DOI: 10.1093/nar/19.8.1917 PMC Full Text View journal details
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