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PMID: 170611 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Relationship of the first step in protein synthesis to ppGpp: formation of A(5')ppp(5')Gpp.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2653-7

Rapaport E, Svihovec SK, Zamecnik PC

Abstract

In the presence of purified Escherichia coli lysyl-tRNA synthetase [L-lysine:tRNALys ligase (AMP-forming) EC 6.1.1.6], L-lysine, and ATP, addition of the nucleotide ppGpp results in formation of a unique product-A(5')ppp(5') Gpp. The same compound is also formed very rapidly in a cell-free protein-synthesizing system when ppGpp is added. The possible significance of this reaction in the rapid turnover of ppGpp and as a more general mechanism by which an AMP residue is activated and introduced onto a 5'-diphosphorylated species, including the 5'-end of an RNA, is further discussed.

MeSH Terms
Adenine Nucleotides/metabolism Adenosine Triphosphate/metabolism Alkaline Phosphatase Avian Myeloblastosis Virus/metabolism Bacterial Proteins/biosynthesis Chromatography, DEAE-Cellulose Chromatography, Thin Layer Escherichia coli/metabolism Guanine Nucleotides/metabolism Lysine-tRNA Ligase/metabolism Phosphoric Diester Hydrolases Protein Biosynthesis RNA, Viral/metabolism Snake Venoms Transfer RNA Aminoacylation
Chemicals
Adenine Nucleotides Bacterial Proteins Guanine Nucleotides RNA, Viral Snake Venoms Adenosine Triphosphate Alkaline Phosphatase Phosphoric Diester Hydrolases Lysine-tRNA Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rapaport E
Svihovec S K
Zamecnik P C
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2653
Published
1975-07-00
Pages
2653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432828
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2653 PMC Full Text View journal details
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