Reversibility of the pyrophosphoryl transfer from ATP to GTP by Escherichia coli stringent factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3470-3

Sy J

Abstract

The stringent factor-catalyzed, ribosome-dependent synthesis of guanosine polyphosphates is found to be reversible. The reverse reaction specifically requires 5'-AMP as the pyrophosphoryl acceptor, and guanosine 5'-triphosphate-3'-diphosphate is preferentially utilized as the pyrophosphoryl donor. The primary products of the reaction are GTP and ATP. The reverse reaction is strongly inhibited by the antibiotics thiostrepton and tetracycline, and by ATP and beta-gamma-methylene-adenosine-triphosphate, but not by ADP, GTP, and GDP. The reverse reaction occurs under conditions for nonribosomal synthesis. The overall reaction for stringent factor-catalyzed guanosine polyphosphate formation may thus be formulated: (p)pp5'G + ppp5'A right harpoon over left harpoon (p)pp5'G3'pp + p5'A.

MeSH Terms

Adenosine Diphosphate/pharmacology Adenosine Monophosphate/metabolism Adenosine Triphosphate/analogs & derivatives,pharmacology Anti-Bacterial Agents/pharmacology Chromatography, DEAE-Cellulose Escherichia coli/enzymology Guanine Nucleotides/metabolism,pharmacology Guanosine Triphosphate/analogs & derivatives,metabolism,pharmacology Peptides/pharmacology Phosphoric Acids Phosphotransferases/metabolism Tetracycline/pharmacology

Chemicals

Anti-Bacterial Agents Guanine Nucleotides Peptides Phosphoric Acids Adenosine Monophosphate Adenosine Diphosphate Guanosine Triphosphate Adenosine Triphosphate Phosphotransferases Tetracycline

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Sy J

References (10)

10 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.9.3470

Published

1974-09-00

Pages

3470-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433795

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.9.3470 PMC Full Text View journal details

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Reversibility of the pyrophosphoryl transfer from ATP to GTP by Escherichia coli stringent factor.

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