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PMID: 17050737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.

Molecular biology of the cell ·Vol. 17 ·No. 12 ·2006-12-00 ·Pages 5356-71

Shimada M, Kanematsu K, Tanaka K, Yokosawa H, Kawahara H

Abstract

The ubiquitin-binding RPN-10 protein serves as a ubiquitin receptor that delivers client proteins to the 26S proteasome. Although ubiquitin recognition is an essential step for proteasomal destruction, deletion of the rpn-10 gene in yeast does not influence viability, indicating redundancy of the substrate delivery pathway. However, their specificity and biological relevance in higher eukaryotes is still enigmatic. We report herein that knockdown of the rpn-10 gene, but not any other proteasome subunit genes, sexually transforms hermaphrodites to females by eliminating hermaphrodite spermatogenesis in Caenorhabditis elegans. The feminization phenotype induced by deletion of the rpn-10 gene was rescued by knockdown of tra-2, one of sexual fate decision genes promoting female development, and its downstream target tra-1, indicating that the TRA-2-mediated sex determination pathway is crucial for the Delta rpn-10-induced sterile phenotype. Intriguingly, we found that co-knockdown of rpn-10 and functionally related ubiquitin ligase ufd-2 overcomes the germline-musculinizing effect of fem-3(gf). Furthermore, TRA-2 proteins accumulated in rpn-10-defective worms. Our results show that the RPN-10-mediated ubiquitin pathway is indispensable for control of the TRA-2-mediated sex-determining pathway.

MeSH Terms
Alleles Animals Base Sequence Caenorhabditis elegans/cytology,metabolism Caenorhabditis elegans Proteins/genetics,metabolism Exons/genetics Female Feminization Gene Deletion Infertility Intestinal Mucosa/metabolism Male Membrane Proteins/metabolism Molecular Sequence Data Mutant Proteins/metabolism Phenotype Proteasome Endopeptidase Complex/metabolism Receptors, Cytoplasmic and Nuclear/genetics,metabolism Sex Determination Processes Spermatogenesis/physiology
Chemicals
Caenorhabditis elegans Proteins Membrane Proteins Mutant Proteins RPN-10 protein, C elegans Receptors, Cytoplasmic and Nuclear fem-3 protein, C elegans tra-2 protein, C elegans Proteasome Endopeptidase Complex ATP dependent 26S protease
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shimada Masumi
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Kanematsu Kenji
Tanaka Keiji
Yokosawa Hideyoshi
Kawahara Hiroyuki
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-05-0437
Published
2006-12-00
Epub
2006-00-18
Pages
5356-71
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1687211
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e06-05-0437 PMC Full Text View journal details
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