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PMID: 17028203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The tomato NBARC-LRR protein Prf interacts with Pto kinase in vivo to regulate specific plant immunity.

The Plant cell ·Vol. 18 ·No. 10 ·2006-10-00 ·Pages 2792-806

Mucyn TS, Clemente A, Andriotis VM, Balmuth AL, Oldroyd GE, Staskawicz BJ, Rathjen JP

Abstract

Immunity in tomato (Solanum lycopersicum) to Pseudomonas syringae bacteria expressing the effector proteins AvrPto and AvrPtoB requires both Pto kinase and the NBARC-LRR (for nucleotide binding domain shared by Apaf-1, certain R gene products, and CED-4 fused to C-terminal leucine-rich repeats) protein Prf. Pto plays a direct role in effector recognition within the host cytoplasm, but the role of Prf is unknown. We show that Pto and Prf are coincident in the signal transduction pathway that controls ligand-independent signaling. Pto and Prf associate in a coregulatory interaction that requires Pto kinase activity and N-myristoylation for signaling. Pto interacts with a unique Prf N-terminal domain outside of the NBARC-LRR domain and resides in a high molecular weight recognition complex dependent on the presence of Prf. In this complex, both Pto and Prf contribute to specific recognition of AvrPtoB. The data suggest that the role of Pto is confined to the regulation of Prf and that the bacterial effectors have evolved to target this coregulatory molecular switch.

MeSH Terms
Base Sequence DNA Primers Lycopersicon esculentum/enzymology,immunology,metabolism,microbiology Molecular Sequence Data Molecular Weight Plant Proteins/metabolism Protein Binding Protein Serine-Threonine Kinases/metabolism Pseudomonas syringae/pathogenicity Signal Transduction Tobacco/metabolism
Chemicals
DNA Primers Plant Proteins Prf protein, Lycopersicon esculentum Pto protein, Lycopersicon Protein Serine-Threonine Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mucyn Tatiana S
The Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom.
Clemente Alfonso
Andriotis Vasilios M E
Balmuth Alexi L
Oldroyd Giles E D
Staskawicz Brian J
Rathjen John P
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.044016
Published
2006-10-00
Epub
2006-00-06
Pages
2792-806
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1626632
Subset
IM
Databases
GENBANK
DQ019170, DQ374448, DQ374449, DQ374450, U65391
External Links
PubMed source DOI: 10.1105/tpc.106.044016 PMC Full Text View journal details
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