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PMID: 17021849 Published · ppublish English Journal Article

Regulation of the beta-hydroxyacyl ACP dehydratase gene of Picea mariana by alternative splicing.

Plant cell reports ·Vol. 26 ·No. 1 ·2007-01-00 ·Pages 105-13

Tai HH, Williams M, Iyengar A, Yeates J, Beardmore T

Abstract

The gene for beta-hydroxyacyl ACP dehydratase, a de novo fatty acid biosynthetic enzyme, was cloned from Picea mariana (black spruce) and consists of five exons and four introns. The first intron of the beta-hydroxyacyl ACP dehydratase mRNA is alternatively spliced. Retention of intron 1 in splice variants results in truncation of the beta-hydroxyacyl ACP dehydratase ORF at a premature termination codon. In addition, splicing of intron 1 was found to be associated with cold temperature. mRNAs retaining intron 1 increase with seed imbibition at 22 degrees C but not 4 degrees C, whereas, splicing of intron 1 increases in winter weeks with temperatures below freezing. These results provide evidence that alternative splicing may also contribute to regulation of lipid biosynthesis in Picea mariana.

MeSH Terms
Alternative Splicing/genetics Amino Acid Sequence Base Sequence Blotting, Northern Enoyl-CoA Hydratase/genetics Exons/genetics Gene Expression Regulation, Plant Introns/genetics Molecular Sequence Data Picea/enzymology,genetics Plant Proteins/genetics RNA Splice Sites/genetics RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction/methods Seasons Seeds/enzymology,genetics Sequence Alignment Temperature
Chemicals
Plant Proteins RNA Splice Sites RNA, Messenger Enoyl-CoA Hydratase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tai Helen H
Canadian Forest Service, Natural Resources Canada, P.O. Box 4000, Fredericton, NB, E3B 5P7, Canada. htai@nrcan.gc.ca
Williams Martin
Iyengar Abhinav
Yeates Jessica
Beardmore Tannis
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Article Info
Journal
Plant cell reports
Abbr.
Plant Cell Rep
ISSN
0721-7714
DOI
10.1007/s00299-006-0213-7
Email
htai@nrcan.gc.ca
Published
2007-01-00
Epub
2006-00-05
Pages
105-13
Language
English
Region
Germany
NLM ID
9880970
Subset
IM
Databases
GENBANK
DQ173272, DQ173273
External Links
PubMed source DOI: 10.1007/s00299-006-0213-7 View journal details
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