Home LiteratureArticle Details

PMID: 16959888 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Microbial community in a sediment-hosted CO2 lake of the southern Okinawa Trough hydrothermal system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 38 ·2006-09-19 ·Pages 14164-9

Inagaki F, Kuypers MM, Tsunogai U, Ishibashi J, Nakamura K, Treude T, Ohkubo S, Nakaseama M, Gena K, Chiba H, Hirayama H, Nunoura T, Takai K, Jørgensen BB, Horikoshi K, Boetius A

Abstract

Increasing levels of CO2 in the atmosphere are expected to cause climatic change with negative effects on the earth's ecosystems and human society. Consequently, a variety of CO2 disposal options are discussed, including injection into the deep ocean. Because the dissolution of CO2 in seawater will decrease ambient pH considerably, negative consequences for deep-water ecosystems have been predicted. Hence, ecosystems associated with natural CO2 reservoirs in the deep sea, and the dynamics of gaseous, liquid, and solid CO2 in such environments, are of great interest to science and society. We report here a biogeochemical and microbiological characterization of a microbial community inhabiting deep-sea sediments overlying a natural CO2 lake at the Yonaguni Knoll IV hydrothermal field, southern Okinawa Trough. We found high abundances (>10(9) cm(-3)) of microbial cells in sediment pavements above the CO2 lake, decreasing to strikingly low cell numbers (10(7) cm(-3)) at the liquid CO2/CO2-hydrate interface. The key groups in these sediments were as follows: (i) the anaerobic methanotrophic archaea ANME-2c and the Eel-2 group of Deltaproteobacteria and (ii) sulfur-metabolizing chemolithotrophs within the Gamma- and Epsilonproteobacteria. The detection of functional genes related to one-carbon assimilation and the presence of highly 13C-depleted archaeal and bacterial lipid biomarkers suggest that microorganisms assimilating CO2 and/or CH4 dominate the liquid CO2 and CO2-hydrate-bearing sediments. Clearly, the Yonaguni Knoll is an exceptional natural laboratory for the study of consequences of CO2 disposal as well as of natural CO2 reservoirs as potential microbial habitats on early Earth and other celestial bodies.

MeSH Terms

Archaea/classification,genetics,metabolism Biomarkers/chemistry,metabolism Carbon Dioxide/chemistry,metabolism Ecosystem Environment Geologic Sediments/chemistry,microbiology Humans Lipids/chemistry Molecular Sequence Data Pacific Ocean Phylogeny Seawater/chemistry Water Microbiology

Chemicals

Biomarkers Lipids Carbon Dioxide

Authors & Affiliations

16 authors, click to expand affiliations / ORCID

Inagaki Fumio

Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka 237-0061, Japan. inagaki@jamstec.go.jp

Kuypers Marcel M M

Tsunogai Urumu

Ishibashi Jun-Ichiro

Nakamura Ko-Ichi

Treude Tina

Ohkubo Satoru

Nakaseama Miwako

Gena Kaul

Chiba Hitoshi

Hirayama Hisako

Nunoura Takuro

Takai Ken

Jørgensen Bo B

Horikoshi Koki

Boetius Antje

References (22)

22 references, click to expand

Direct experiments on the ocean disposal of fossil fuel CO2
Science. 1999 May 7;284(5416):943-5 PMID: 10320370
Biogeographical distribution and diversity of microbes in methane hydrate-bearing deep marine sediments on the Pacific Ocean Margin.
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2815-20 PMID: 16477011
Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance.
Microbiol Rev. 1991 Jun;55(2):288-302 PMID: 1886522
Sulfurovum lithotrophicum gen. nov., sp. nov., a novel sulfur-oxidizing chemolithoautotroph within the epsilon-Proteobacteria isolated from Okinawa Trough hydrothermal sediments.
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1477-82 PMID: 15388698
Variability in microbial community and venting chemistry in a sediment-hosted backarc hydrothermal system: Impacts of subseafloor phase-separation.
FEMS Microbiol Ecol. 2005 Sep 1;54(1):141-55 PMID: 16329980
Venting of carbon dioxide-rich fluid and hydrate formation in mid-okinawa trough backarc basin.
Science. 1990 Jun 1;248(4959):1093-6 PMID: 17733370
Identification of methyl coenzyme M reductase A (mcrA) genes associated with methane-oxidizing archaea.
Appl Environ Microbiol. 2003 Sep;69(9):5483-91 PMID: 12957937
Enzymatic and genetic characterization of carbon and energy metabolisms by deep-sea hydrothermal chemolithoautotrophic isolates of Epsilonproteobacteria.
Appl Environ Microbiol. 2005 Nov;71(11):7310-20 PMID: 16269773
Rapid detection and quantification of members of the archaeal community by quantitative PCR using fluorogenic probes.
Appl Environ Microbiol. 2000 Nov;66(11):5066-72 PMID: 11055964
Molecular fossil record of elevated methane levels in late Pleistocene coastal waters.
Science. 2003 Feb 21;299(5610):1214-7 PMID: 12595688
Evolution of carbon sinks in a changing climate.
Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11201-6 PMID: 16061800
Radiative habitable zones in martian polar environments.
Icarus. 2005 Jun;175(2):360-71 PMID: 16044598
Methane-consuming archaebacteria in marine sediments.
Nature. 1999 Apr 29;398(6730):802-5 PMID: 10235261
Diversity and distribution of methanotrophic archaea at cold seeps.
Appl Environ Microbiol. 2005 Jan;71(1):467-79 PMID: 15640223
Novel chemoautotrophic endosymbiosis between a member of the Epsilonproteobacteria and the hydrothermal-vent gastropod Alviniconcha aff. hessleri (Gastropoda: Provannidae) from the Indian Ocean.
Appl Environ Microbiol. 2005 Sep;71(9):5440-50 PMID: 16151136
Field studies on the formation of sinking CO2 particles for ocean carbon sequestration: effects of injector geometry on particle density and dissolution rate and model simulation of plume behavior.
Environ Sci Technol. 2005 Sep 15;39(18):7287-93 PMID: 16201660
Microbial diversity of hydrothermal sediments in the Guaymas Basin: evidence for anaerobic methanotrophic communities.
Appl Environ Microbiol. 2002 Apr;68(4):1994-2007 PMID: 11916723
Biomarker evidence for widespread anaerobic methane oxidation in Mediterranean sediments by a consortium of methanogenic archaea and bacteria. The Medinaut Shipboard Scientific Party.
Appl Environ Microbiol. 2000 Mar;66(3):1126-32 PMID: 10698781
Perennial water ice identified in the south polar cap of Mars.
Nature. 2004 Apr 8;428(6983):627-30 PMID: 15024393
Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments.
Appl Environ Microbiol. 2001 Apr;67(4):1922-34 PMID: 11282650
A marine microbial consortium apparently mediating anaerobic oxidation of methane.
Nature. 2000 Oct 5;407(6804):623-6 PMID: 11034209
Characterization of C1-metabolizing prokaryotic communities in methane seep habitats at the Kuroshima Knoll, southern Ryukyu Arc, by analyzing pmoA, mmoX, mxaF, mcrA, and 16S rRNA genes.
Appl Environ Microbiol. 2004 Dec;70(12):7445-55 PMID: 15574947

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.0606083103

inagaki@jamstec.go.jp

Published

2006-09-19

Epub

2006-00-07

Pages

14164-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC1599929

Subset

Databases

GENBANK

AB252422, AB252423, AB252424, AB252425, AB252426, AB252427, AB252428, AB252429, AB252430, AB252431, AB252432, AB252433, AB252434, AB252435, AB252436, AB252437, AB252438, AB252439, AB252440, AB252441, AB252442, AB252443, AB252444, AB252445, AB252446, AB252447, AB252448, AB252449, AB252450, AB252451, AB252452, AB252453, AB252454, AB252455

Corrections

CommentIn

PMID: 16971489

External Links

PubMed source DOI: 10.1073/pnas.0606083103 PMC Full Text View journal details

Analysis Services

Microbial community in a sediment-hosted CO2 lake of the southern Okinawa Trough hydrothermal system.

Contact

WeChat Official Account