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PMID: 11282650 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments.

Applied and environmental microbiology ·Vol. 67 ·No. 4 ·2001-04-00 ·Pages 1922-34

Orphan VJ, Hinrichs KU, Ussler W, Paull CK, Taylor LT, Sylva SP, Hayes JM, Delong EF

Abstract

The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant (13)C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. (13)C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta-proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong (13)C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant (13)C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.

MeSH Terms
Anaerobiosis Archaea/classification,genetics,metabolism DNA, Ribosomal/analysis,genetics Geologic Sediments/microbiology In Situ Hybridization, Fluorescence Lipids/analysis Methane/metabolism Molecular Sequence Data Oxidation-Reduction Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Seawater/microbiology Sequence Analysis, DNA Sulfates/metabolism Sulfur-Reducing Bacteria/classification,genetics,metabolism
Chemicals
DNA, Ribosomal Lipids RNA, Ribosomal, 16S Sulfates Methane
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Orphan V J
Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd., P.O. Box 628, Moss Landing, California 95039. orphan@mbari.org
Hinrichs K U
Ussler W
Paull C K
Taylor L T
Sylva S P
Hayes J M
Delong E F
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.4.1922-1934.2001
Email
orphan@mbari.org
Published
2001-04-00
Pages
1922-34
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC92814
Subset
IM
Databases
GENBANK
AF354126, AF354127, AF354128, AF354129, AF354130, AF354131, AF354132, AF354133, AF354134, AF354135, AF354136, AF354137, AF354138, AF354139, AF354140, AF354141, AF354142, AF354143, AF354144, AF354145, AF354146, AF354147, AF354148, AF354149, AF354150, AF354151, AF354152, AF354153, AF354154, AF354155, AF354156, AF354157, AF354158, AF354159, AF354160, AF354161, AF354162, AF354163, AF354164, AF354165, AF354166, AF354167
External Links
PubMed source DOI: 10.1128/AEM.67.4.1922-1934.2001 PMC Full Text View journal details
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