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PMID: 16957816 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates.

Histochemistry and cell biology ·Vol. 127 ·No. 2 ·2007-02-00 ·Pages 139-48

Kahle M, Pridalová J, Spacek M, Dzijak R, Hozák P

Abstract

Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cell Nucleus/metabolism Conserved Sequence Female Gene Expression Humans Male Mice Myosin Type I/chemistry,genetics,isolation & purification,metabolism Nucleic Acid Amplification Techniques Phylogeny Sequence Homology, Nucleic Acid Serum/chemistry Transcription, Genetic Vertebrates/genetics,metabolism
Chemicals
Myosin Type I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kahle M
Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular Biology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20, Prague 4, Czech Republic.
Pridalová J
Spacek M
Dzijak R
Hozák P
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Article Info
Journal
Histochemistry and cell biology
Abbr.
Histochem Cell Biol
ISSN
0948-6143
DOI
10.1007/s00418-006-0231-0
Published
2007-02-00
Epub
2006-00-07
Pages
139-48
Language
English
Region
Germany
NLM ID
9506663
Subset
IM
External Links
PubMed source DOI: 10.1007/s00418-006-0231-0 View journal details
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