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PMID: 16951073 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.

Genetics ·Vol. 174 ·No. 2 ·2006-10-00 ·Pages 1057-61

Luce AC, Sharma A, Mollere OS, Wolfgruber TK, Nagaki K, Jiang J, Presting GG, Dawe RK

Abstract

Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

MeSH Terms

Centromere/genetics Chromatin/genetics Chromosome Mapping Genetic Markers Immunoprecipitation Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Zea mays/genetics

Chemicals

Chromatin Genetic Markers

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Luce Amy C

Department of Plant Biology, University of Georgia, Georgia 30602, USA.

Sharma Anupma

Mollere Oliver S B

Wolfgruber Thomas K

Nagaki Kiyotaka

Jiang Jiming

Presting Gernot G

Dawe R Kelly

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Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1534/genetics.106.060467

Published

2006-10-00

Epub

2006-00-01

Pages

1057-61

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1602074

Subset

External Links

PubMed source DOI: 10.1534/genetics.106.060467 PMC Full Text View journal details

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Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.

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