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PMID: 16938467 Published · ppublish English Journal Article

Atomic resolution structures in nuclear transport.

Methods (San Diego, Calif.) ·Vol. 39 ·No. 4 ·2006-08-00 ·Pages 342-55

Süel KE, Cansizoglu AE, Chook YM

Abstract

There are currently at least 53 structures of components of nuclear transport in the Protein Databank. In addition to providing critical insights into molecular mechanisms of nuclear transport, these atomic resolution structures provide a large body of information that could guide biochemical and cell biological analyses involving nuclear transport proteins. This paper catalogs 53 crystal and NMR structures of nuclear transport proteins, with the emphasis on providing information useful for mutagenesis and overexpression of recombinant proteins.

MeSH Terms
Active Transport, Cell Nucleus Amino Acids Biological Transport, Active Cell Nucleus/physiology Crystallography, X-Ray Karyopherins/chemistry Models, Molecular Nuclear Magnetic Resonance, Biomolecular Protein Conformation
Chemicals
Amino Acids Karyopherins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Süel Katherine E
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, TX 75390-9041, USA.
Cansizoglu Ahmet E
Chook Yuh Min
References (54)
54 references, click to expand
  1. GLFG and FxFG nucleoporins bind to overlapping sites on importin-beta.
    J Biol Chem. 2002 Dec 27;277(52):50597-606 PMID: 12372823
  2. The three-dimensional structure of the autoproteolytic, nuclear pore-targeting domain of the human nucleoporin Nup98.
    Mol Cell. 2002 Aug;10(2):347-58 PMID: 12191480
  3. Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats.
    EMBO J. 2002 Jun 17;21(12):2843-53 PMID: 12065398
  4. Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta.
    Mol Cell. 2002 Dec;10(6):1345-53 PMID: 12504010
  5. Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution.
    J Mol Biol. 2003 Feb 21;326(3):849-58 PMID: 12581645
  6. Synergy of silent and hot spot mutations in importin beta reveals a dynamic mechanism for recognition of a nuclear localization signal.
    J Biol Chem. 2003 May 2;278(18):16216-21 PMID: 12594203
  7. Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15.
    EMBO Rep. 2003 Jul;4(7):699-703 PMID: 12835756
  8. Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha.
    J Biol Chem. 2003 Jul 25;278(30):27981-7 PMID: 12695505
  9. Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-alpha.
    Biochem J. 2003 Oct 15;375(Pt 2):339-49 PMID: 12852786
  10. Structural basis for Nup2p function in cargo release and karyopherin recycling in nuclear import.
    EMBO J. 2003 Oct 15;22(20):5358-69 PMID: 14532109
  11. The structure of importin-beta bound to SREBP-2: nuclear import of a transcription factor.
    Science. 2003 Nov 28;302(5650):1571-5 PMID: 14645851
  12. Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
    Cell. 1984 Jul;37(3):801-13 PMID: 6086146
  13. Sequence requirements for nuclear location of simian virus 40 large-T antigen.
    Nature. 1984 Sep 6-11;311(5981):33-8 PMID: 6088992
  14. Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen.
    Mol Cell Biol. 1986 Nov;6(11):4136-9 PMID: 3025638
  15. The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2).
    J Mol Biol. 1996 Jul 19;260(3):422-31 PMID: 8757804
  16. Conserved histidine residues of RCC1 are essential for nucleotide exchange on Ran.
    J Biochem. 1996 Jul;120(1):82-91 PMID: 8864848
  17. Mutations within the Ran/TC4 GTPase. Effects on regulatory factor interactions and subcellular localization.
    J Biol Chem. 1996 Dec 20;271(51):32834-41 PMID: 8955121
  18. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran.
    J Mol Biol. 1997 Oct 10;272(5):716-30 PMID: 9368653
  19. The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller.
    Nature. 1998 Mar 5;392(6671):97-101 PMID: 9510255
  20. Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran.
    J Mol Biol. 1998 Apr 3;277(3):635-46 PMID: 9533885
  21. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha.
    Cell. 1998 Jul 24;94(2):193-204 PMID: 9695948
  22. The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2).
    J Mol Biol. 1998 Dec 18;284(5):1517-27 PMID: 9878368
  23. Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport.
    Nature. 1999 Mar 4;398(6722):39-46 PMID: 10078529
  24. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha.
    Nat Struct Biol. 1999 Apr;6(4):388-97 PMID: 10201409
  25. Structure of importin-beta bound to the IBB domain of importin-alpha.
    Nature. 1999 May 20;399(6733):221-9 PMID: 10353244
  26. Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp.
    Nature. 1999 May 20;399(6733):230-7 PMID: 10353245
  27. Structural view of the Ran-Importin beta interaction at 2.3 A resolution.
    Cell. 1999 May 28;97(5):635-46 PMID: 10367892
  28. Model of the ran-RCC1 interaction using biochemical and docking experiments.
    J Mol Biol. 1999 Jun 18;289(4):1119-30 PMID: 10369786
  29. The crystal structure of rna1p: a new fold for a GTPase-activating protein.
    Mol Cell. 1999 Jun;3(6):781-91 PMID: 10394366
  30. Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex.
    J Cell Biol. 2004 Nov 22;167(4):591-7 PMID: 15557116
  31. The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.
    Mol Cell. 2004 Dec 3;16(5):749-60 PMID: 15574330
  32. Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex.
    Mol Cell. 2004 Dec 3;16(5):761-75 PMID: 15574331
  33. Structural basis for the assembly of a nuclear export complex.
    Nature. 2004 Dec 16;432(7019):872-7 PMID: 15602554
  34. Phospholipid scramblase 1 contains a nonclassical nuclear localization signal with unique binding site in importin alpha.
    J Biol Chem. 2005 Mar 18;280(11):10599-606 PMID: 15611084
  35. Solution structure of the Ran-binding domain 2 of RanBP2 and its interaction with the C terminus of Ran.
    J Mol Biol. 2005 May 6;348(3):711-25 PMID: 15826666
  36. The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding.
    Mol Cell. 2005 Apr 29;18(3):355-67 PMID: 15866177
  37. Structural basis for the high-affinity binding of nucleoporin Nup1p to the Saccharomyces cerevisiae importin-beta homologue, Kap95p.
    J Mol Biol. 2005 Jun 10;349(3):515-25 PMID: 15878174
  38. Structural basis for nuclear import complex dissociation by RanGTP.
    Nature. 2005 Jun 2;435(7042):693-6 PMID: 15864302
  39. Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex.
    Nature. 2005 Jun 2;435(7042):687-92 PMID: 15931224
  40. Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p.
    J Biol Chem. 2005 Oct 21;280(42):35723-32 PMID: 16105837
  41. Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling.
    EMBO J. 2005 Nov 2;24(21):3681-9 PMID: 16222336
  42. Rules for nuclear localization sequence recognition by karyopherin beta 2.
    Cell. 2006 Aug 11;126(3):543-58 PMID: 16901787
  43. Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP.
    J Mol Biol. 1999 Oct 29;293(3):579-93 PMID: 10543952
  44. Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha.
    Structure. 2000 Mar 15;8(3):329-38 PMID: 10745017
  45. Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha.
    J Mol Biol. 2000 Apr 14;297(5):1183-94 PMID: 10764582
  46. Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
    Cell. 2000 Jul 7;102(1):99-108 PMID: 10929717
  47. The adoption of a twisted structure of importin-beta is essential for the protein-protein interaction required for nuclear transport.
    J Mol Biol. 2000 Sep 8;302(1):251-64 PMID: 10964573
  48. The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain.
    EMBO J. 2000 Nov 1;19(21):5587-98 PMID: 11060011
  49. Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).
    Cell. 2001 Apr 20;105(2):245-55 PMID: 11336674
  50. Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor.
    Mol Cell. 2001 Sep;8(3):645-56 PMID: 11583626
  51. RanGAP mediates GTP hydrolysis without an arginine finger.
    Nature. 2002 Feb 7;415(6872):662-6 PMID: 11832950
  52. Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1.
    Cell. 2002 Feb 8;108(3):345-56 PMID: 11853669
  53. The crystal structure and mutational analysis of a novel RNA-binding domain found in the human Tap nuclear mRNA export factor.
    Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1888-93 PMID: 11854490
  54. Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1.
    Nat Struct Biol. 2002 Apr;9(4):247-51 PMID: 11875519
Article Info
Journal
Methods (San Diego, Calif.)
Abbr.
Methods
ISSN
1046-2023
DOI
10.1016/j.ymeth.2006.06.015
Published
2006-08-00
Pages
342-55
Language
English
Region
United States
NLM ID
9426302
PMCID
PMC3471385
Subset
IM
Grants
NIGMS NIH HHS · R01 GM069909 · United States
External Links
PubMed source DOI: 10.1016/j.ymeth.2006.06.015 PMC Full Text View journal details
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