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PMID: 1691707 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cyclic hair-loss and regrowth in transgenic mice overexpressing an intermediate filament gene.

The EMBO journal ·Vol. 9 ·No. 5 ·1990-05-00 ·Pages 1485-93

Powell BC, Rogers GE

Abstract

We have produced transgenic mice containing up to 250 copies of a sheep wool intermediate filament keratin gene to study the effect of its expression on hair structure and development. Several transgenic lines expressed the gene and in the one containing 250 transgenes, a pattern of hair-loss and regrowth was stably established. Successive waves of hair growth follow periods of denuding like the natural progression of hairs in the mouse hair cycle. By in situ hybridization we have shown that the sheep transgenes are expressed at the correct stage in mouse hair development and at a high level. The transgenic hairs contain not only an elevated level of intermediate filament keratin protein but also a decreased level of the filament-associated proteins. This imbalance disrupts the normal ordered array of these proteins in the cells of the hair cortex and leads to weakened fibres which are prematurely lost.

MeSH Terms
Amino Acids/analysis Animals Hair/abnormalities,growth & development Intermediate Filament Proteins/biosynthesis,genetics Keratins/biosynthesis,genetics Mice Mice, Transgenic Microscopy, Electron Mutation Nucleic Acid Hybridization RNA Probes Restriction Mapping Sheep Wool/growth & development
Chemicals
Amino Acids Intermediate Filament Proteins RNA Probes Keratins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Powell B C
Adelaide University Centre for Gene Technology, Department of Biochemistry, University of Adelaide, South Australia.
Rogers G E
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-05-00
Pages
1485-93
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551839
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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